Pneumocystis jirovecii Real-time PCR Kit (MD02451)

Activity: Target: Kexin-like serine protease (kex1) gene

999,00

150 reactions

999,00

Description: NZYTech Real-time PCR Kit for Pneumocystis jirovecii is designed for the in vitro quantification of P. jirovecii genomes. The kit is designed to have the broadest detection profile possible whilst remaining specific to the P. jirovecii genome. Our kit for P. jirovecii has been designed for the specific and exclusive in vitro quantification of the P. jirovecii species whitin human samples. Please note that this species has recently been reclassified and was previously known as P. carinii f. sp. Hominis. The target sequence within the KEX1 gene has previously been shown to be a good genetic marker for P. jirovecii in other clinical real-time PCR based studies (Rohner P., et al., 2009). This gene has no known homology with other species in the P. carinii at the primer binding sites. As such, the primers and probe sequences in this kit have 100% homology with a broad range of clinically relevant reference sequences based on a comprehensive bioinformatics analysis. The kit includes positive control for copy number determination, as well as an internal extraction template to control the quality of the nucleic acids extraction avoiding false negative results.

Features:
– Nucleic acid target: DNA
– Robust Lyophilized qPCR master mix
– PCR Method: quantitative PCR
– Detection Method: Primer-Probe Detection
– Rapid detection of clinically relevant subtypes
– Positive copy number quantification
– Highly specific detection profile
– High priming efficiency
– Sensitive to < 100 copies of target - Accurate controls - For research use only

Components:
– Specific primer/probe mix – FAM labelled
– Positive control template – for Standard curve
– Internal extraction control primer/probe mix – VIC labelled
– Internal extraction control
– Endogenous control primer/probe mix – FAM labelled
– Lyo NZYSupreme qPCR master mix (2x)
– Lyo reference dye

Storage conditions: -20 °C

Shipping conditions: Room temperature

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