Description: NZYTech Monkeypox virus Real-time PCR Kit, RUO includes all reagents required to identify the presence of monkeypox virus DNA in a variety of relevant samples. DNA isolated and purified from different sample sources is amplified in a single reaction using two highly specific primers/probe sets. The probes specifically anneal to two regions of the monkeypox virus genome, namely B7R and CrmB genes. An additional primers/probe set acts as an endogenous internal control to detect nucleic acids of β-actin.
Features:
For research use only;
Triplex target assay (Monkeypox virus B7R and CrmB genes and human β-actin gene);
Positive control to validate assay included;
Internal control to confirm extraction / inhibition included
Aplications:
This test is intended for the in vitro qualitative detection of monkeypox virus genome. A positive result indicates the presence of Monkeypox virus DNA. This kit is intended for use by laboratory trained personnel, specifically instructed in real-time PCR techniques.
Specifications:
High Sensitivity
High Throughput: up to 93 samples (96-well plate)
High Specificity: proven by in silico analysis
Flexibility: designed to be used with a variety of qPCR instruments
Fast Time: < 50 min from nucleic acid extraction to results
Minimal sample input: As low as 2 μL sample needed
Components:
NZYSupreme qPCR Probe Master Mix (2x)
Monkeypox virus (B7R & CrmB genes)/ACTB PPMix (FAM™, HEX™ and Cy5™ labelled)
No-Template Control
Monkeypox virus (B7R & CrmB genes) /ACTB Positive Control
Storage conditions: -30 °C to -15 °C
Shipping conditions: Dry Ice to Blue Ice
