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SKU
MD03191

SARS-CoV-2 One-Step RT-PCR Kit

Storage Conditions:
-85 °C to -15 °C
One-step real-time RT-PCR is the fastest and most reliable method to perform an accurate detection of SARS-CoV-2 RNA, which is indicative of an human infection.
Availability
Ready to Ship
Blue Ice / Wet Ice
Unit Size
96 reactions
€599.00
One-step real-time RT-PCR is the fastest and most reliable method to perform an accurate detection of SARS-CoV-2 RNA, which is indicative of an human infection. NZYtech SARS-CoV-2 One-Step RT-PCR Kit includes all reagents required to identify the presence of SARS-CoV-2 RNA in a variety of relevant samples. RNA isolated and purified from different biological material is retrotranscribed to cDNA and subsquently amplified in a single reaction using two highly specific primers/probe sets exploiting the so-called TaqMan® principle. During this process, one probe specifically anneals to one region of SARS-CoV-2 RdRp gene, in case the sample was extracted from an infected patient. An additional primer/probe set acts as an internal control to detect nucleic acids of the human RNase P gene (RP), which allows confirming the efficacy of the RNA extraction process from human derived biological material. In addition, this internal control allows to demonstrate that no reaction inhibition has occurred by PCR inhibitors potencially present in the clinical/environmental samples. The two probes are differently labelled, with FAM and JOE reporter dyes, respectively, to allow identifying the individual amplification of the two gene targets in a single reaction. In addition, they are provided in optimized concentrations to make sure amplification of human mRNA, even when present at very high concentrations, do not limit the efficiency of the SARS-CoV-2 primers/probe set. In case SARS-CoV-2 quantification is required a SARS-CoV-2 Copy Number Quantification Nucleic Acid is provided at 1 x 10^7 copies/μL.
More Information
Shipping Conditions Blue Ice
Storage Conditions -85 °C to -15 °C
Format Liquid
Product Category Predesigned qPCR assays
Compatibility All standard Real-time thermal cyclers
Target Species SARS-CoV-2
Sample Material RNA
Protocol time Approx. 60 min (from extracted NA to result)
Number of targets Singleplex
Internal Control No
Detection Method Probe Based
- NZYSpeedy One-step RT-qPCR Master Mix
- SARS-CoV-2/RP primers/probe mix (FAM/JOE labelled, respectively)
- SARS-CoV-2/RP Positive Control
- SARS-CoV-2 Copy Number Quantification Nucleic Acid
- RNase/DNase free Water
More Information
Shipping Conditions Blue Ice
Storage Conditions -85 °C to -15 °C
Format Liquid
Product Category Predesigned qPCR assays
Compatibility All standard Real-time thermal cyclers
Target Species SARS-CoV-2
Sample Material RNA
Protocol time Approx. 60 min (from extracted NA to result)
Number of targets Singleplex
Internal Control No
Detection Method Probe Based
Molecular Beacon Assay Development for Severe Acute Respiratory Syndrome Coronavirus 2 Detection.Carvalho J, Lopes-Nunes J, Figueiredo J, Santos T, Miranda A, Riscado M, Sousa F, Duarte AP, Socorro S, Tomaz CT, Felgueiras M, Teixeira R, Faria C, Cruz C.  Sensors. 2021; 21(21):7015. https://doi.org/10.3390/s21217015
More Information
Shipping Conditions Blue Ice
Storage Conditions -85 °C to -15 °C
Format Liquid
Product Category Predesigned qPCR assays
Compatibility All standard Real-time thermal cyclers
Target Species SARS-CoV-2
Sample Material RNA
Protocol time Approx. 60 min (from extracted NA to result)
Number of targets Singleplex
Internal Control No
Detection Method Probe Based
More Information
Shipping Conditions Blue Ice
Storage Conditions -85 °C to -15 °C
Format Liquid
Product Category Predesigned qPCR assays
Compatibility All standard Real-time thermal cyclers
Target Species SARS-CoV-2
Sample Material RNA
Protocol time Approx. 60 min (from extracted NA to result)
Number of targets Singleplex
Internal Control No
Detection Method Probe Based

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