For Research use only
Description: One-step real-time RT-PCR is the fastest and most reliable method to perform an accurate detection of SARS-CoV-2 RNA. NZYTech’s Veterinary SARS-CoV-2 One-Step RT-qPCR Kit (RUO) includes all reagents required to detect the presence of SARS-CoV-2 RNA in a variety of animal samples. Extracted and purified RNA from different biological material is reverse transcribed to cDNA and subsequently amplified in a single reaction using two highly specific primers/probe sets exploiting the so-called TaqMan® principle. During this process, one probe specifically anneals to a region of SARS-CoV-2 N gene, when the sample was extracted from an infected animal, and an additional primers/probe set acts as an internal control to detect β-actin (ACTB gene) nucleic acids. The internal control detection validate the efficacy of the extraction process and the absence of PCR inhibitors potencially present in the animals biological samples. This internal control detection was validated for a variety of animal species. The two probes are differently fluorescence labelled, with FAM and HEX reporter dyes, respectively, to allow the identification of the two target genes in a single reaction. In addition, they are provided in optimized concentrations to make sure amplification of animal mRNA, even when present at very high concentrations, does not limit the efficiency of the SARS-CoV-2 primers/probe set.
Please check the list of the suitable species to be tested with this kit.
-NZYSupreme One-step RT-qPCR Master Mix
-SARS-CoV-2/ACTB primers/probe mix (FAM/HEX labelled, respectively)
-SARS-CoV-2/ACTB Positive Control (1 x 104 copies/μL)
-RNase/DNase free Water
Storage conditions: -30 °C to -15 °C
Shipping conditions: Dry Ice to Blue Ice