Custom Gene Synthesis (GS003)

0,24129,00

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Quotes and Orders:

Please download our Order Form, complete and send it to services@nzytech.com.
You will receive your project and quote within 24h.

 

Description:
NZYTech’s Custom Gene Synthesis service provides in vitro chemical synthesis, cloning and 100% sequence verification of any desired DNA genetic sequence. NZYTech’s R&D department improved standard molecular biology processes and developed innovative bio-informatics tools for gene design and codon optimization. Our proprietary gene optimization algorithm – ATGenium – comprehensively optimizes critical factors in transcription, translation and co-translational protein folding to deliver highest levels of expression in any host system. Genes are cloned into our standard cloning vectors. Downstream services, including sub-cloning, site directed mutagenesis, plasmid prep and protein expression are available upon request.

Features:
– Free gene design & codon optimization
– Flexible gene design process: addition of tags, restriction sites or other elements
– 100% sequence accuracy guaranteed
– Cloning vector – pUC57 (Ampicillin/Kanamycin)
– Sub-cloning into any destination vector
– Complete confidentiality guaranteed

Delivery Specifications:
– 0.5-1 μg of purified plasmid
– Gene Synthesis Report

Codon optimization of your genes

Proteins play a variety of critical roles in many biological processes. They are valuable therapeutic compounds, research reagents and industrial catalysts. NZYTech’s proprietary codon optimization software – ATGenium – can alter both naturally occurring and recombinant gene sequences to achieve the highest possible levels of protein expression in any given expression system. This will save you time and money, while getting you quickly to meaningful results. ATGenium takes into consideration a variety of critical factors involved in different stages of protein expression, such as codon adaptability, mRNA structure, and various cis-elements in transcription and translation. Optimizing your gene sequence will have significant advantages in a variety of applications such as recombinant protein expression, DNA vaccine design and protein engineering.

Optimizing heterologous protein production in Escherichia coli with ATGenium:
Superior levels of recombinant proteins expressed in Escherichia coli strains can be achieved through codon optimization using the NZYTech’s ATGenium algorithm.

ATGenium_expression_levels

A. Levels of protein expression of three different eukaryotic proteins in a prokaryotic system (Escherichia coli). The genes encoding these proteins were optimized by three algorithms for codons optimization, by two different competitors (light blue) and using the ATGenium algorithm developed by NZYTech (dark blue).

B. High-throughput recombinant protein expression of CAZYmes (Carbohydrate Active eNZymes). Genes encoding these proteins were optimized using the ATGenium algorithm, cloned into pHTP1 expression vector and overexpressed incoli BL21 (DE3) strain. Recombinant proteins were purified through IMAC and separated by SDS-PAGE. High protein expression yields were obtained when genes were optimized using the ATGenium algorithm

1. Why should I use NZYTech’s gene synthesis services?
– You can focus on your research while we produce your gene
– It is possible to codon optimize your gene, thus increasing the possibility of achieving high protein levels
– Gene synthesis provides access to DNA templates that are not easily available on nature

2. Is it possible to synthesize sequences with high/low GC content or repeats?
Yes, NZYTech successfully synthesizes complex genes.

3. Is it possible to optimize gene design for high levels of expression?
Yes, ATGenium allows optimizing not only critical factors in transcription and translation but also factors related with co-translational protein folding. ATGenium comprises an extensive collection of codon usage tables, allowing optimizing codon usage for improved protein expression in different host systems.

4. Are all genes sequence-verified?
All genes are 100% sequence verified.

5. In which vector am I going to receive my gene?
You will receive your gene cloned into our standard cloning vectors (pUC57 – Ampicilin/Kanamycin).

6. Is it possible to sub-clone my gene into a commonly used expression vector?
Yes, we can sub-clone your gene into your vector of choice. Alternatively, your synthetic gene can be easily transferred from the entry vector to any of our pHTP E. coli expression vectors for a very competitive price.

7. How do you deliver synthetic genes?
NZYTech delivers 0.5 to 1 µg of plasmid DNA.

8. What is NZYTech’s policy for Intellectual Property Protection?
NZYTech does not claim rights or ownership of any intellectual property related to the DNA/amino acid sequences provided by our customers or the resulting synthetic genes. No data or material will be released to a third party without your consent. All genes resulting from gene synthesis and/or cloning services are stored at NZYTech for a three months period. After this period nucleic acids provided to customers are discarded.

The effect of an engineered ATCUN motif on the structure and biophysical properties of the SH3 domain of c-Src tyrosine kinase
Plaza-Garrido M, Salinas-García MC, Cámara-Artigas A
J Biol Inorg Chem, 2020

Physiological characterization of a pyrimidine auxotroph exposes link between uracil phosphoribosyltransferase regulation and riboflavin production in Ashbya gossypii
Silva R, Aguiar TQ, Oliveira C, Domingues L
N Biotechnol, 2019

Molecular Recognition of a Thomsen–Friedenreich Antigen Mimetic Targeting Human Galectin‐3
Santarsia S, Grosso AS, Trovão F, Jiménez-Barbero J, Carvalho AL, Nativi C, Marcelo F 
ChemMedChem, 2018

Protein-Glycan Quinary Interactions in Crowding Environment Unveiled by NMR Spectroscopy
Diniz A, Dias JS, Jiménez-Barbero J, Marcelo F, Cabrita EJ 
Chemistry, 2017

Engineering Corynebacterium glutamicum for the production of 2,3-butanediol
Rados D, Carvalho AL, Wieschalka S, Neves AR, Blombach B, Eikmanns BJ, Santos H 
Microbial Cell Factories, 2015

Production of curcuminoids from tyrosine by a metabolically engineered Escherichia coli using caffeic acid as an intermediate.
Rodrigues JL, Araújo RG, Prather KL, Kluskens LD, Rodrigues LR
Biotechnol J, 2015

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