High-Throughput Gene Synthesis (HS0010)

Price list (orders > 48 genes):

Description: NZYTech’s R&D team has recently developed a High-throughput (HTP) Gene Synthesis platform to efficiently generate hundreds to thousands of synthetic genes. With that in mind, we have improved standard molecular biology processes and developed innovative bio-informatics tools for gene design and codon optimization. We offer a high quality HTP Gene Synthesis service for DNA fragments < than 4 kb. HTP Gene Synthesis will allow you to tackle difficult research projects such as those involving determining structure-function relationships in different proteins, creating novel antibody libraries, screening gene variant libraries or optimizing protein function and expression. Our codon optimization technology – ATGenium – will allow obtaining the highest possible levels of gene expression in different expressions hosts while retaining high levels of protein solubility.

HTP Gene Synthesis_workflow

– Free gene design & codon optimization – ATGenium – DNA sequences can be optimized to improve protein expression in different hosts
– Flexible, custom process: sequences can be engineered to add tags, restriction sites, promoters or other elements
– All genes are 100% sequence verified
– Synthetic genes are cloned into our standard cloning vectors (pHTP0 or other) or into any expression vector from our pHTP library
– Minimum orders of 48 genes
– Saves you time and money
– Quality assurance certificate
– Absolute confidentiality is guaranteed

Complementary services (*)
– Your synthetic gene can be easily transferred from the standard entry vectors to our pHTP Escherichia coli expression plasmids for a very competitive price (see gene cloning). High protein expression levels can be reached when using pHTP expression vectors (see expression levels).
– Screening the best expression conditions for your recombinant protein is also available and may include gene sub-cloning into a battery of pHTP expression vectors (see figure below), followed by protein expression and purification (see enhanced protein expression)

(*) at additional price

Your synthetic genes can be easily transferred from the standard entry vector to our pHTP Escherichia coli expression plasmids for a very competitive price. Price for sub-cloning into one of NZYTech’s pHTP expression vectors is 65 €. High protein expression is reached when using this system (please check data in Figures & Tables section). In addition, we can screen the best expression conditions for your protein using a battery of proprietary designed destination vectors.


Synthetic genes cloned into pHTP0 cloning vector can be easily transferred into any pHTP E. coli expression vector. The portfolio of pHTP expression vectors includes a different range of fusion tags, thus offering the possibility of quickly assaying levels of expression and solubility of a desired protein in multiple conditions.

Delivery Specifications:
– 0.5-1 μg of purified recombinant plasmids containing your synthetic genes (in 96-well plates)
– HTP Gene Synthesis Report containing vector map, gene sequences and QC certificate
– Estimated delivery: depends on each project complexity

Quotes & Ordering:
For quotations and orders, please email us to services@nzytech.com. Upload and fill out the NZYTech’s HTS Order Form.

Additional information:
– Your gene sequences will be reviewed by our experience scientists in order to evaluate the presence of elements that may interfere with synthesis and/or assembly performance. Design warnings include:

– Secondary structure: hairpins; local and global GC content
– Repeat content; homopolymers: A/C/T <=10 bases and G <=6 bases
– Restriction sites

– You will receive a HTP gene synthesis project describing your detailed request. In case your gene sequence does not pass the screening criteria, our gene services specialists will contact you to determine the best way to proceed.
– Downstream services also available upon request (gene sub-cloning; site-directed mutagenesis & recombinant protein production)
– Beware that higher DNA yields and endotoxin free DNA may be provided upon request and with an additional cost
– Please notice that each gene will be screened to identify potentially dangerous pathogen sequences. NZYTech reserves the right to refuse any order that does not pass this biosafety screen.

pHTP0 vector map
pUC57 vector map
Order Form
Codon optimization of your genes

Proteins play a variety of critical roles in many biological processes. They are valuable therapeutic compounds, research reagents and industrial catalysts. NZYTech’s proprietary codon optimization software – ATGenium – can alter both naturally occurring and recombinant gene sequences to achieve the highest possible levels of protein expression in any given expression system. This will save you time and money, while getting you quickly to meaningful results. ATGenium takes into consideration a variety of critical factors involved in different stages of protein expression, such as codon adaptability, mRNA structure, and various cis-elements in transcription and translation. Optimizing your gene sequence will have significant advantages in a variety of applications such as recombinant protein expression, DNA vaccine design and protein engineering.

Optimizing heterologous protein production in Escherichia coli with ATGenium:
Superior levels of recombinant proteins expressed in Escherichia coli strains can be achieved through codon optimization using the NZYTech’s ATGenium algorithm.


A. Levels of protein expression of three different eukaryotic proteins in a prokaryotic system (Escherichia coli). The genes encoding these proteins were optimized by three algorithms for codons optimization, by two different competitors (light blue) and using the ATGenium algorithm developed by NZYTech (dark blue).

B. High-throughput recombinant protein expression of CAZYmes (Carbohydrate Active eNZymes). Genes encoding these proteins were optimized using ATGenium algorithm and overexpressed in Escherichia coli BL21 (DE3). Recombinant proteins were purified through IMAC and separated by SDS-PAGE. High protein expression yields were obtained when genes were optimized using ATGenium algorithm.

1. Why should I use NZYTech’s gene synthesis services?
The key advantages of producing genes synthetically are the following:

– Less expensive than traditional cloning.
– You can spend your time on other projects while your gene is being produced.
– It is possible to codon optimize your gene, thus increasing the possibility of achieving high protein levels.
– Most DNA sequences can be synthesized.
– Gene synthesis provides access to DNA templates that are not easily available on nature.

2. Is it possible to synthesize sequences with high GC content or repeats?
Sequences with high and low GC content are complex to be handled and usually require a longer production period. However, NZYTech has successfully synthesized genes with high/low GC contents. Please contact us to carefully review every sequence; we will provide you a custom gene synthesis project, which includes turnaround time and sequence details.

3. Is it possible to optimize gene design for high levels of expression?
ATGenium algorithm optimizes different factors that are known to affect mRNA stability, such as GC content and secondary structures. It allows optimizing not only critical factors in transcription and translation but also factors related with co-translational protein folding. The relative frequency of codon usage varies widely depending on the organism. ATGenium comprises an extensive collection of codon usage tables, allowing optimizing codon usage for improved protein expression in different host systems.

4. In which vector am I going to receive my gene?
If you have not requested sub-cloning of your gene into a specific vector, you will receive your gene cloned into our standard cloning vector pHTP0 or other. Our cloning vectors are ampicillin resistant. Vector maps are available on the website. We can easily transfer your gene into one or more pHTP expression vector(s) for a very convenient price.

5. Are all genes sequence-verified?
All genes are 100% sequence verified.

6. Is it possible to sub-clone my gene into a commonly used expression vector?
Your synthetic gene can be easily transferred from the entry vector to any of our pHTP E. coli expression vectors for a very competitive price. Alternatively, we can sub-clone your gene into your vector of choice.

7. How do you deliver synthetic genes?
NZYTech delivers 0.5 to 1 µg of plasmid DNA.

8. What is NZYTech’s policy for Intellectual Property Protection?
NZYTech does not claim rights or ownership of any intellectual property related to the DNA/amino acid sequences provided by our customers or the resulting synthetic genes. No data or material will be released to a third party without your consent. All genes resulting from gene synthesis and/or cloning services are stored at NZYTech for a three months period. After this period nucleic acids provided to customers are discarded.

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