High-Throughput Gene Cloning (HS00201)

50,00

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Quotes and Orders:

Please download our Order Form, complete and send it to services@nzytech.com.
You will receive your project and quote within 24h.

 

Description:
NZYTech offers a high-throughput cloning service for large projects, typically involving several hundreds to thousands of genes. A proprietary method is used for cloning your genes into one or more selected vectors. Our method does not require the use of conventional restriction enzymes and DNA ligases, which increases speed while improving cloning efficiencies, as only the desired DNA fragments are cloned into the destination vector(s) in the correct orientation.

HTP gene cloning_workflow

A DNA template source provided by the customer will be used for gene amplification, by PCR. Alternatively, genes can be chemically synthesized (at an additional cost). DNA fragments are cloned into standard cloning vectors, and this cloning method allows to easily exchange genes from an entry vector (pHTP0) into various pHTP Escherichia coli expression plasmids (see figure below). NZYTech has an own library of several pHTP expression plasmids containing different tags, known to enhance protein’s solubility and/or promote correct protein’s folding. This strategy may constitute the best alternative for selecting the most appropriate tag for each protein.

GC_vector_tabelapHTP

PCR fragments or synthetic genes cloned into an entry vector can be easily transferred into any pHTP E. coli expression vector. The NZYTech’s portfolio of pHTP expression vectors includes a different range of fusion tags, thus offering the possibility to quickly assay levels of expression and solubility of a desired protein in multiple conditions.

Features:
– Genes of interest are obtained from gDNA, cDNA, pDNA, phage, cosmid DNA or, alternatively, by gene synthesis
– Fast cloning procedure
– QC by verification of correct plasmids sequence and frame using Sanger sequencing
– Flexibility: gene sub-cloning can be easily achieved from the pHTP0 cloning vector to any of the pHTP derivatives with different fusion tags
– High protein expression levels can be reached when using pHTP expression vectors (see expression levels)
– Considerable saving of time and money
– Customers retain all rights to the sequence data and related intellectual property
– Absolute confidentiality is guaranteed

Complementary services (*):
– Genes can be obtained by HTP Gene Synthesis
Enhanced Protein Expression: screening of the best expression conditions for your recombinant protein, using our battery of pHTP expression vectors. This service includes gene sub-cloning into a battery of pHTP vectors, followed by protein expression and purification.

(*) at additional price

Delivery Specifications:
– 0.5-1 μg of purified recombinant plasmids containing your genes (in 96-well plates)
– HTP Gene Cloning Report: includes QC, vector map and insert DNA sequences
– Estimated delivery: upon request

“The NZYTech High Throughput service is the best choice that you can make if you need the cloning and expression of an high number of enzyme-coding genes in a short time. What is great is dealing with an efficient company and great scientists that assist you in your needs personally along the project up to very end.”
Academic from Italian University

 

“I have used NZYTech’s HTP service on multiple occasions to produce large repertoires that target complex glycans. the service was efficient, the delivery of the recombinant plasmids was prompt and it saved a great postdoc time. This cost effective service has really helped to push our science forward.”
Academic from British University

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