High-Throughput Gene Synthesis (HS005)

0,18109,00

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Quotes and Orders:

Please download our Order Form, complete and send it to services@nzytech.com.
You will receive your project and quote within 24h.

 

Description:
NZYTech’s R&D team developed a High-throughput (HTP) Gene Synthesis platform to efficiently generate hundreds to thousands of synthetic genes. With that in mind, we have improved standard molecular biology processes and developed innovative bioinformatics tools for gene design and codon optimization. HTP Gene Synthesis will allow you to tackle difficult research projects such as those involving determining structure-function relationships in different proteins, creating novel antibody libraries, screening gene variant libraries or optimizing protein function and expression. Our codon optimization technology – ATGenium – will allow obtaining the highest possible levels of gene expression in different expressions hosts while retaining high levels of protein solubility.

Features:
– Free gene design & codon optimization
– Flexible gene design process: addition of tags, restriction sites or other elements
– 100% sequence accuracy guaranteed
– Cloning into standard cloning vectors – pUC57 (Ampicillin/Kanamycin resistance)
– Sub-cloning into any E. coli expression vector from our pHTP library*
– Complete confidentiality guaranteed
(*at an additional price)

Delivery Specifications:
– 0.5-1 μg of purified plasmids
– Gene Synthesis Report

High-throughput Gene Synthesis PipelineHTP Gene Synthesis_workflow
Codon optimization of your genes

Proteins play a variety of critical roles in many biological processes. They are valuable therapeutic compounds, research reagents and industrial catalysts. NZYTech’s proprietary codon optimization software – ATGenium – can alter both naturally occurring and recombinant gene sequences to achieve the highest possible levels of protein expression in any given expression system. This will save you time and money, while getting you quickly to meaningful results. ATGenium takes into consideration a variety of critical factors involved in different stages of protein expression, such as codon adaptability, mRNA structure, and various cis-elements in transcription and translation. Optimizing your gene sequence will have significant advantages in a variety of applications such as recombinant protein expression, DNA vaccine design and protein engineering.

Optimizing heterologous protein production in Escherichia coli with ATGenium:
Superior levels of recombinant proteins expressed in Escherichia coli strains can be achieved through codon optimization using the NZYTech’s ATGenium algorithm.

ATGenium_expression_levels

A. Levels of protein expression of three different eukaryotic proteins in a prokaryotic system (Escherichia coli). The genes encoding these proteins were optimized by three algorithms for codons optimization, by two different competitors (light blue) and using the ATGenium algorithm developed by NZYTech (dark blue).

B. High-throughput recombinant protein expression of CAZYmes (Carbohydrate Active eNZymes). Genes encoding these proteins were optimized using ATGenium algorithm and overexpressed in Escherichia coli BL21 (DE3). Recombinant proteins were purified through IMAC and separated by SDS-PAGE. High protein expression yields were obtained when genes were optimized using ATGenium algorithm.

1. Why should I use NZYTech’s gene synthesis services?
– You can focus on your research while we produce your gene
– It is possible to codon optimize your gene, thus increasing the possibility of achieving high protein levels
– Gene synthesis provides access to DNA templates that are not easily available on nature

2. Is it possible to synthesize sequences with high/low GC content or repeats?
Yes, NZYTech successfully synthesizes complex genes.

3. Is it possible to optimize gene design for high levels of expression?
Yes, ATGenium allows optimizing not only critical factors in transcription and translation but also factors related with co-translational protein folding. ATGenium comprises an extensive collection of codon usage tables, allowing optimizing codon usage for improved protein expression in different host systems.

4. Are all genes sequence-verified?
All genes are 100% sequence verified.

5. In which vector am I going to receive my gene?
You will receive your gene cloned into our standard cloning vectors (pUC57 – Ampicilin/Kanamycin).

6. Is it possible to sub-clone my gene into an expression vector?
Yes, your synthetic gene can be easily transferred from the entry vector to any of our pHTP E. coli expression vectors for a very competitive price.

7. How do you deliver synthetic genes?
NZYTech delivers 0.5 to 1 µg of plasmid DNA.

8. What is NZYTech’s policy for Intellectual Property Protection?
NZYTech does not claim rights or ownership of any intellectual property related to the DNA/amino acid sequences provided by our customers or the resulting synthetic genes. No data or material will be released to a third party without your consent. All genes resulting from gene synthesis and/or cloning services are stored at NZYTech for a three months period. After this period nucleic acids provided to customers are discarded.

“The NZYTech High Throughput service is the best choice that you can make if you need the cloning and expression of an high number of enzyme-coding genes in a short time. What is great is dealing with an efficient company and great scientists that assist you in your needs personally along the project up to very end.”
Academic from Italian University

 

“I have used NZYTech’s HTP service on multiple occasions to produce large repertoires that target complex glycans. the service was efficient, the delivery of the recombinant plasmids was prompt and it saved a great postdoc time. This cost effective service has really helped to push our science forward.”
Academic from British University

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