Oligo synthesis

Description: NZYTech delivers oligos produced following rigorous quality control checks with fast turnaround times that are suitable for most Molecular Biology applications such PCR, qPCR, sequencing, hybridization and antisense studies. Additionally, NZYTech delivers an extensive series of modifications.

Delivery Specifications:
-five different synthesis scales: 10 nmol, 50 nmol, 200 nmol, 1000 nmol and 10000 nmol
-purification options: Salt Free, HPSF, HPLC and PAGE
-sequence length: 5 – 120 bases (5 – 50 for the 10 nmol) for unmodified oligos; 5 – 80 for modified oligos
-quality control by OD measurement and MALDI-TOF MS

Quotes & Ordering:
To order NZYTech Oligos & Probes, please download the Order Template Form and send it as an Excel attachment to info@nzytech.com. The order form also includes guidelines for ordering.

Oligos Order Template Form

Get a Free Discount Coupon if your paper refers NZYTech Products!

If NZYTech’s products are mentioned in a paper published by your lab, you will be rewarded with a Free Discount Coupon of 50 € to use in any order. Please note that the final paper/page proof (as PDF files) or a downloadable link must be provided so NZYTech can confirm that your submission is valid for this promotion.

See more details here.

 

Featured citations

The 17-mer oligonucleotides 5’-TAMRA-TC ACC *TC GTA CGA CTC-3’ and 3’-BHQ-2-AG TGG GAG CAT GCT GAG-5’ (in which TAMRA is the fluorophore carboxytetramethylrhodamine, BHQ-2 is black hole quencher-2 and * is tetrahydrofuran, a stable abasic site analogue) (35) were custom-made by NZYTech (Lisbon, Portugal).
Guerreiro, P.S., Estácio, S.G., Antunes, F., Fernandes, A.S., Pinheiro, P.F., Costa, J.G., Castro, M., Miranda, J.P., Guedes, R.C., Oliveira, N.G. (2016). Structure-based virtual screening towards the discovery of novel inhibitors of the DNA repair activity of the human apurin ic/apyrimidinic endonuclease 1. Chem Biol Drug Des 88(6):915-925.

 

Primers and reagents used for PCR amplifications were purchased from NZYTech Lda (Lisbon, Portugal).
Oliveira, M., Tavares, M, Gomes, D., Touret, T., Braz, B. S., Tavares, L., Semedo-Lemsaddek, T. (2016). Virulence traits and antibiotic resistance among enterococci isolated from dogs with periodontal disease. Comparative Immunology, Microbiology and Infectious Diseases 46:27-31

 

The sequence of the signal peptide derived from the small tetra-heme cytochrome c (STC) from S. oneidensis MR-1 was used to produce a chimeric gene with therJR_0333 gene from T. potens using the primers listed in Table 1 (NZYtech, Portugal)
Costa, N. L., Carlson, H. K., Coates, J. D., Louro, R. O., Paquete, C. M. (2015). Heterologous expression and purification of a multiheme cytochrome from a Gram-positive bacterium capable of performing extracellular respiration. Protein Expression and Purification 111: 48-52

 

The primer sequences were designed and synthesized by NZYTech and are described in Table 2. All measurements were performed in triplicate. A single melting peak was obtained for each gene analyzed in all samples.
Menino, J. F., Saraiva, M., Gomes-Rezende, J., Sturme, M., Pedrosa, J., et al. (2013) P. brasiliensis Virulence Is Affected by SconC, the Negative Regulator of Inorganic Sulfur Assimilation. PLoS ONE 8(9): e74725

You may also like…