Protein Production (MS004)

400,002.150,00

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Due to Covid19 and the social distancing measures adopted, we are currently not accepting orders for this service.

Quotes and Orders:

Please download our Order Form, complete and send it to services@nzytech.com.
You will receive your project and quote within 24h.

 

Standard Protocol:
– Starting material: plasmid DNA provided by the customer or previously obtained at NZYTech through Gene synthesis and/or Gene Cloning services
– Expression system: E. coli BL21 (DE3)
– Recombinant proteins purified through Immobilized Metal Affinity Chromatography (IMAC)
– SDS-PAGE analysis & protein quantification

Deliverables:
– Protein expression report
– 1-2 mg of purified recombinant protein

 

Enhanced Protocol – Stage 1Small-scale expression screening:
– Target gene amplification (gene synthesis at an additional price)
– Cloning into 12 pHTP E. coli expression vectors containing different tags known to enhance protein’s solubility and/or promote correct protein’s folding
– Expression system: E. coli BL21 (DE3) – Small-scale protein production trials: different growth media and induction temperatures
– Recombinant protein purification through Immobilized Metal Affinity Chromatography (IMAC)
– SDS-PAGE analysis & protein quantification

Deliverables:
– Protein Expression Report
– Recombinant plasmids
– Recombinant soluble proteins obtained with success (150 µL)

 

Enhanced Protocol – Stage 2Large-scale protein production:
– Scale-up recombinant protein expression using optimized conditions
– Recombinant proteins purified through IMAC
– SDS-PAGE analysis & protein quantification

Deliverables:
– Protein Production Report
– 1-2 mg of purified recombinant proteins
– In case of not being possible to proceed with stage 2, all charges related to stage 1 will still be applicable

 

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