Borrelia burgdorferi sensu lato qPCR Kit

Borrelia burgdorferi sensu lato is a group of gram-negative bacteria primarily transmitted by the bite of infected ticks. These group is composed by Borrelia burgdorferi sensu stricto, B. garinii, B. afzelii, B. valaisiana, B. lusitaniae and B. spielmanii. These bacteria are responsible for causing Lyme disease in humans, a multisystemic illness characterized by symptoms such as fever, rash, and joint pain. Borrelia burgdorferi sensu lato qPCR Kit is designed for the in vitro detection of Borrelia species belonging to the Borrelia burgdorferi sensu lato complex.

The kit is built to have the broadest possible detection profile whilst remaining specific to B. burgdorferi sensu lato. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of B. burgdorferi sensu lato genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database at the time of design.

This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used. If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see https://nzytech.com/en/molecular-diagnostics/). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.