Brachyspira hyodysenteriae & Brachyspira pilosicoli qPCR Kit

Brachyspira hyodysenteriae and Brachyspira pilosicoli are Gram-negative anaerobic bacteria and are the causative agents of swine dysentery (SD) and porcine intestinal spirochetosis (PIS), respectively. While SD is characterized by mucohemorrhagic diarrhea and inflammation in the large intestine, PIS is characterized by mild colitis, diarrhea, reduced pigs growth rate and, consequently, increased time to market weight.

Brachyspira hyodysenteriae & Brachyspira pilosicoli qPCR Kit is designed for the in vitro detection of B. hyodysenteriae and B. pilosicoli genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to B. hyodysenteriae and B. pilosicoli. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of these species. The primers and probe sequences have very high (>95%) homology with a broad range of B. hyodysenteriae and B. pilosicoli genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected.

This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.