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SKU
MD06081
Campylobacter spp. qPCR Kit
Designed for the in vitro detection of Campylobacter spp. qPCR assay criteria are met, but PC is not meant for quantification.
Availability
Ready to Ship
Campylobacter is a Gram-negative, microaerophilic genus of bacteria of the family Campylobacteriaceae. It colonizes the digestive system of a broad range of animals, including poultry, pigs and cattle and it is the most common bacterial food-borne pathogen worldwide causing light to severe diarrhoea. Although there are more than 20 species of Campylobacter, not all of them cause human illness. Approximately 90% of human Campylobacter illness is caused by Campylobacter jejuni. Less common species, such as C. coli, C. upsaliensis, C. fetus, and C. lari, can also infect humans.
Campylobacter spp. qPCR Kit is designed for the in vitro detection of Campylobacter spp. genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to Campylobacter spp. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this genus. The primers and probe sequences have very high (>95%) homology with a broad range of Campylobacter spp. genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related genus are not detected.
This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.
If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available for purchase (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Campylobacter spp. qPCR Kit is designed for the in vitro detection of Campylobacter spp. genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to Campylobacter spp. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this genus. The primers and probe sequences have very high (>95%) homology with a broad range of Campylobacter spp. genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related genus are not detected.
This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.
If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available for purchase (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Campylobacter species |
Sample Material | DNA |
Protocol time | Approx. 60 min. (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Detection Method | Probe Based |
- Lyo NZYSupreme qPCR master mix (2x)
- qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- DNA Internal Extraction Control (IEC)
- qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- DNA Internal Extraction Control (IEC)
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Campylobacter species |
Sample Material | DNA |
Protocol time | Approx. 60 min. (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Detection Method | Probe Based |
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Campylobacter species |
Sample Material | DNA |
Protocol time | Approx. 60 min. (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Detection Method | Probe Based |
MSDS
Material Safety Data Sheets
CoA
Certificate of Analysis