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Rhamnogalacturonan endolyase 11A, Cellvibrio japonicus
Rhamnogalacturonan endolyase 11A (CjRge11A), assigned the E.C. number 4.2.2.23, is a derivative of Cellvibrio japonicus. It is an enzyme that participates in the endotype eliminative cleavage of L-α-rhamnopyranosyl-1,4-α-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end. The recombinant CjRge11A, purified from Escherichia coli, is a single-domain Pectate Lyase family 11 (PL11) enzyme (see more details at www.cazy.org).
Rhamnogalacturonan endolyase 11A (CjRge11A), assigned the E.C. number 4.2.2.23, is a derivative of Cellvibrio japonicus. It is an enzyme that participates in the endotype eliminative cleavage of L-α-rhamnopyranosyl-1,4-α-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end. The recombinant CjRge11A, purified from Escherichia coli, is a single-domain Pectate Lyase family 11 (PL11) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 0,25 mg/mL. Bulk quantities of this product can be made available upon request. To place an order, simply visit our website. We offer fast and secure shipping worldwide.
Shipping Conditions | Shipping: Dry Ice to RT |
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Storage Conditions | -30°C to -15°C |
Activity | Rhamnogalacturonan lyases |
Family | PL11 |
EC number | 4.2.2.23 |
Organism | Cellvibrio japonicus |
Architecture | PL11 |
Optimal pH | 9.5 |
Optimal Temperature | 45°C |
Substrates | rhamnogalacturonans |
McKie et al. (2001) Biochemical Journal 355, 167-177.
Shipping Conditions | Shipping: Dry Ice to RT |
---|---|
Storage Conditions | -30°C to -15°C |
Activity | Rhamnogalacturonan lyases |
Family | PL11 |
EC number | 4.2.2.23 |
Organism | Cellvibrio japonicus |
Architecture | PL11 |
Optimal pH | 9.5 |
Optimal Temperature | 45°C |
Substrates | rhamnogalacturonans |
Shipping Conditions | Shipping: Dry Ice to RT |
---|---|
Storage Conditions | -30°C to -15°C |
Activity | Rhamnogalacturonan lyases |
Family | PL11 |
EC number | 4.2.2.23 |
Organism | Cellvibrio japonicus |
Architecture | PL11 |
Optimal pH | 9.5 |
Optimal Temperature | 45°C |
Substrates | rhamnogalacturonans |
CoA
Certificate of Analysis