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SKU
MD05461
Class A Beta-lactamase (blaGES) resistance qPCR Kit
Designed for the in vitro detection of AR-blaGES. qPCR assay criteria are met, but PC is not meant for quantification.
Availability
Ready to Ship
Antibiotic resistance is one of the biggest threats to global health, food security, and development. New resistance mechanisms are emerging and spreading globally, threatening the ability to treat common infectious diseases. A growing list of infections, such as pneumonia, tuberculosis, blood poisoning, gonorrhoea, and foodborne diseases, are becoming harder to treat as antibiotics become less effective.
Class A Beta-lactamase (blaGES) resistance qPCR Kit is designed for the in vitro detection of Class A Beta-lactamase blaGES genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to blaGES genes. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of these sequences. The primers and probe sequences have very high (>95%) homology with a broad range of blaGES sequences based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. The kit will detect the blaGES gene from all clinically relevant strains.
This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.
If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Class A Beta-lactamase (blaGES) resistance qPCR Kit is designed for the in vitro detection of Class A Beta-lactamase blaGES genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to blaGES genes. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of these sequences. The primers and probe sequences have very high (>95%) homology with a broad range of blaGES sequences based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. The kit will detect the blaGES gene from all clinically relevant strains.
This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.
If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Antibiotic resistance blaGES |
Sample Material | DNA |
Protocol time | Approx. 60 min (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Detection Method | Probe Based |
- Lyo NZYSupreme qPCR master mix (2x)
- qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- DNA Internal Extraction Control (IEC)
- qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- DNA Internal Extraction Control (IEC)
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Antibiotic resistance blaGES |
Sample Material | DNA |
Protocol time | Approx. 60 min (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Detection Method | Probe Based |
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Antibiotic resistance blaGES |
Sample Material | DNA |
Protocol time | Approx. 60 min (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Detection Method | Probe Based |
CoA
Certificate of Analysis