Staphylococcus aureus qPCR Kit

Staphylococcus aureus is a Gram-positive spherically shaped bacterium that is a typical member of the microbiota of the body, frequently found in the upper respiratory tract and on the skin. Although S. aureus usually acts as a commensal of the human microbiota, it can also become an opportunistic pathogen, being a common cause of skin infections including abscesses, respiratory infections such as sinusitis, and food poisoning.

Staphylococcus aureus qPCR Kit is designed for the in vitro detection of S. aureus genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to S. aureus. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of S. aureus genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. This kit can also detects the following Staphylococcus species: S. argenteus and S. roterodami. Real-time PCR detection methods have been used in several studies to identify S. aureus genomes (Galia L. et al 2019).

This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Vet, Food & Pharma and Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.