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SKU
MD05201
Equid herpesvirus 4 qPCR Kit
Designed for the in vitro detection of EHV-4. qPCR assay criteria are met, but PC is not meant for quantification.
Availability
Ready to Ship
Equid herpesvirus 4 (EHV-4) is a virus of the family Herpesviridae that cause rhinopneumonitis in horses. It is the most important viral cause of respiratory infection in foals. Like other herpesviruses, EHV-4 causes a lifelong latent infection in affected animals.
Equid herpesvirus 4 qPCR Kit is designed for the in vitro detection of EHV-4 genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to EHV-4. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of EHV-4 genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected. Real-time PCR detection methods have been used in several studies to identify EHV-4 genomes (Diallo I.S. 2007).
This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.
If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Equid herpesvirus 4 qPCR Kit is designed for the in vitro detection of EHV-4 genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to EHV-4. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of EHV-4 genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected. Real-time PCR detection methods have been used in several studies to identify EHV-4 genomes (Diallo I.S. 2007).
This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.
If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Equid herpesvirus 4 |
Sample Material | DNA |
Protocol time | Approx. 60 min. (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Detection Method | Probe Based |
- Lyo NZYSupreme qPCR master mix (2x)
- qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- DNA Internal Extraction Control (IEC)
- qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- DNA Internal Extraction Control (IEC)
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Equid herpesvirus 4 |
Sample Material | DNA |
Protocol time | Approx. 60 min. (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Detection Method | Probe Based |
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Equid herpesvirus 4 |
Sample Material | DNA |
Protocol time | Approx. 60 min. (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Detection Method | Probe Based |
CoA
Certificate of Analysis