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SKU
MD05291
Lyme Disease qPCR Kit
Designed for the in vitro detection of Borrelia spp. qPCR assay criteria are met, but PC is not meant for quantification.
Availability
Ready to Ship
Lyme disease is a vector-borne disease caused by the spirochete bacterium Borrelia. This bacterium is transmitted to humans by the bites of infected ticks of the genus Ixodes.
Lyme Disease qPCR Kit is designed for the in vitro detection of Borrelia species genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to Borrelia spp. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this genus. The primers and probe sequences have very high (>95%) homology with a broad range of Borrelia spp. genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. This kit detects the following Borrelia species that are the most significant causative agents of Lyme disease: B. burgdorferi, B. garinii and B. afzelii. Other Borrelia species and other closely related genus are not detected. Real-time PCR detection methods have been used in several studies to identify Borrelia spp. genomes (Saidac D.S. et al 2009).
This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.
If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Lyme Disease qPCR Kit is designed for the in vitro detection of Borrelia species genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to Borrelia spp. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this genus. The primers and probe sequences have very high (>95%) homology with a broad range of Borrelia spp. genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. This kit detects the following Borrelia species that are the most significant causative agents of Lyme disease: B. burgdorferi, B. garinii and B. afzelii. Other Borrelia species and other closely related genus are not detected. Real-time PCR detection methods have been used in several studies to identify Borrelia spp. genomes (Saidac D.S. et al 2009).
This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.
If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Borrelia species |
Sample Material | DNA |
Protocol time | Approx. 60 min (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Detection Method | Probe Based |
- Lyo NZYSupreme qPCR master mix (2x)
- qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- DNA Internal Extraction Control (IEC)
- qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- DNA Internal Extraction Control (IEC)
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Borrelia species |
Sample Material | DNA |
Protocol time | Approx. 60 min (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Detection Method | Probe Based |
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Borrelia species |
Sample Material | DNA |
Protocol time | Approx. 60 min (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Detection Method | Probe Based |
CoA
Certificate of Analysis