Pseudomonas aeruginosa qPCR Kit

Pseudomonas aeruginosa is a common encapsulated, and facultative anaerobic bacterium that preferentially uses aerobic respiration. P. aeruginosa causes disease in plants and animals, including humans, being a species of considerable medical importance since it is a multidrug resistant pathogen recognized for its ubiquity, its intrinsically advanced antibiotic resistance mechanisms, and its association with serious hospital-acquired infections such as ventilator-associated pneumonia and various sepsis syndromes.

Pseudomonas aeruginosa qPCR Kit is designed for the in vitro detection of P. aeruginosa genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to P. aeruginosa. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of P. aeruginosa genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected. Real-time PCR detection methods have been used in several studies to identify P. aeruginosa genomes (Feizabadi M. M. et al 2010).

This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Vet, Food & Pharma and Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.