Leptospira (LipL32) qPCR Kit

Leptospira is a genus of spirochaete Gram-negative bacteria, which have long, helically coiled cells. This bacterium is spread through the urine of infected animals, which can get into water or soil and can survive for weeks to months. Leptospira is the causative agent of leptospirosis, a disease that affects humans and animals, causing a wide range of symptoms from kidney damage and meningitis to liver failure and respiratory distress.

Leptospirosis spp qPCR Kit is designed for the in vitro detection of Leptospira spp. genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to Leptospira spp. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this genus. Based on a comprehensive bioinformatic analysis with all reference data within the NCBI database, the primers and probe sequences have very high (>95%) homology with Leptospira species. Other closely related genus are not detected. Real-time PCR detection methods have been used in several studies to identify Leptospira spp. genomes (Podgoršek D. et al 2020).

This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Vet, Food & Pharma and Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.