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SKU
MD05871
Leishmania spp qPCR Kit
Designed for the in vitro detection of Leishmania spp. qPCR assay criteria are met, but PC is not meant for quantification.
Availability
Ready to Ship
Leishmania is a parasitic protozoan unicellular eukaryote that is spread through the bite of sandflies of the genus Phlebotomus and Lutzomyia. Leishmania commonly infects hyraxes, canids, rodents, and humans causing leishmaniasis in these organisms.
Leishmania spp qPCR Kit is designed for the in vitro detection of Leishmania spp. genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to Leishmania spp. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this genus. Based on a comprehensive bioinformatic analysis with all reference data within the NCBI database the primers and probe sequences have very high (>95%) homology with a broad range of Leishmania genomes of the following species: Leishmania infantum, Leishmania donovani and Leishmania killicki. Other closely related genus are not detected. Real-time PCR detection methods have been used in several studies to identify Leishmania spp. genomes (Foulet K. et al 2007).
This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.
If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Vet, Food & Pharma and Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Leishmania spp qPCR Kit is designed for the in vitro detection of Leishmania spp. genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to Leishmania spp. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this genus. Based on a comprehensive bioinformatic analysis with all reference data within the NCBI database the primers and probe sequences have very high (>95%) homology with a broad range of Leishmania genomes of the following species: Leishmania infantum, Leishmania donovani and Leishmania killicki. Other closely related genus are not detected. Real-time PCR detection methods have been used in several studies to identify Leishmania spp. genomes (Foulet K. et al 2007).
This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.
If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Vet, Food & Pharma and Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Leishmania species |
Sample Material | DNA |
Protocol time | Approx. 60 min. (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Detection Method | Probe Based |
- Lyo NZYSupreme qPCR master mix (2x)
- qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- DNA Internal Extraction Control (IEC)
- qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- DNA Internal Extraction Control (IEC)
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Leishmania species |
Sample Material | DNA |
Protocol time | Approx. 60 min. (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Detection Method | Probe Based |
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Leishmania species |
Sample Material | DNA |
Protocol time | Approx. 60 min. (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Detection Method | Probe Based |
CoA
Certificate of Analysis