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SKU
MD06071
Brachyspira pilosicoli qPCR Kit
Designed for the in vitro detection of B. pilosicoli. qPCR assay criteria are met, but PC is not meant for quantification.
Availability
Ready to Ship
Brachyspira pilosicoli is a gram-negative, anaerobic, host-associated spirochete that colonizes the intestinal tract of animals and humans. It is the causative agent of intestinal spirochetosis in pigs, chickens and humans. In particular, B. pilosicoli has been described as an important colonic pathogen of pigs and chickens, causing colitis and diarrhea resulting in depressed rates of growth and impaired production on farms where infections with B. pilosicoli may be endemic.
Brachyspira pilosicoli qPCR Kit is designed for the in vitro detection of B. pilosicoli genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to B. pilosicoli. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of B. pilosicoli genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected.
This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.
If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Brachyspira pilosicoli qPCR Kit is designed for the in vitro detection of B. pilosicoli genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to B. pilosicoli. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of B. pilosicoli genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected.
This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.
If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Brachyspira pilosicoli |
Sample Material | DNA |
Protocol time | Approx. 60 min. (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Detection Method | Probe Based |
- Lyo NZYSupreme qPCR master mix (2x)
- qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- DNA Internal Extraction Control (IEC)
- qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- DNA Internal Extraction Control (IEC)
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Brachyspira pilosicoli |
Sample Material | DNA |
Protocol time | Approx. 60 min. (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Detection Method | Probe Based |
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Brachyspira pilosicoli |
Sample Material | DNA |
Protocol time | Approx. 60 min. (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Detection Method | Probe Based |
MSDS
Material Safety Data Sheets
CoA
Certificate of Analysis