Rabbit Haemorrhagic Disease virus 1 & 2 RT-qPCR Kit

Rabbit haemorrhagic disease virus (RHDV) is a positive single-stranded RNA virus classified as a Lagovirus within the /i>Caliciviridae family. It is the causative agent of rabbit haemorrhagic disease (RHD) in adult domesticated and wild rabbits. This disease is extremely contagious, characterized by acute necrotizing hepatitis, but hemorrhages may also be found in other organs, in particular the lungs, heart, and kidneys due to disseminated intravascular coagulation. RHDV has two different serotypes, RHDV-1 and RHDV-2. RHDV-1 may cause death of most or all the rabbits (80–90% lethality) while a variable rate of mortality is observed in rabbits infected by RHDV2 (from 5 to 70%).

Rabbit Haemorrhagic Disease virus 1 & 2 RT-qPCR Kit is designed for the in vitro detection of RHDV genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to RHDV. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of RHDV genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database at the time of design. Due to the inherent instability of RNA viral genomes, it is not possible to guarantee the detection of all clinical isolates.

This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.