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SKU
MD06281

Lumpy Skin Disease virus qPCR Kit

Storage Conditions:
-85 °C to -15 °C
Designed for the in vitro detection of LSDV. qPCR assay criteria are met, but PC is not meant for quantification.
Availability
Ready to Ship
Unit Size
100 reactions
€399.00
Lumpy skin disease virus (LSDV) is a double-stranded DNA virus that belongs to the genus Capripoxvirus within the Poxviridae family. LSDV is the etiologic agent of an infectious disease in cattle, the lumpy skin disease. This disease is characterized by fever, reduced milk production and skin nodules. Other common symptoms are mastitis, swelling of peripheral lymph nodes, loss of appetite, increased nasal discharge and watery eyes. LSDV is one of the most important animal poxviruses because of the serious economic consequences in cattle.

Lumpy Skin Disease virus qPCR Kit is designed for the in vitro detection of LSDV genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to LSDV. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of LSDV genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected.

This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
More Information
Shipping Conditions Room Temperature
Storage Conditions -85 °C to -15 °C
Format Lyophilized
Product Category Predesigned qPCR assays
Compatibility All standard Real-time thermal cyclers
Target Species Lumpy Skin Disease virus
Sample Material RNA
Protocol time Approx. 60 min. (from extracted NA to result)
Number of targets Duplex
Internal Control No
Detection Method Probe Based
- Lyo NZYSupreme One-step RT-qPCR Master Mix (2x)
- RT-qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- RNA Internal Extraction Control (IEC)
More Information
Shipping Conditions Room Temperature
Storage Conditions -85 °C to -15 °C
Format Lyophilized
Product Category Predesigned qPCR assays
Compatibility All standard Real-time thermal cyclers
Target Species Lumpy Skin Disease virus
Sample Material RNA
Protocol time Approx. 60 min. (from extracted NA to result)
Number of targets Duplex
Internal Control No
Detection Method Probe Based
More Information
Shipping Conditions Room Temperature
Storage Conditions -85 °C to -15 °C
Format Lyophilized
Product Category Predesigned qPCR assays
Compatibility All standard Real-time thermal cyclers
Target Species Lumpy Skin Disease virus
Sample Material RNA
Protocol time Approx. 60 min. (from extracted NA to result)
Number of targets Duplex
Internal Control No
Detection Method Probe Based

MSDS

Material Safety Data Sheets

CoA

Certificate of Analysis

Please insert your lot number to get the available CoA

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