We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
SKU
MD06201
Escherichia coli Typing stx1 & stx2 (STEC) qPCR Kit
Designed for the in vitro detection of E. coli stx1 and stx2 genes. qPCR assay criteria are met, but PC is not meant for quantification.
Availability
Ready to Ship
Shiga toxin producing Escherichia coli (STEC) are Gram-negative and facultative anaerobic bacteria. They are foodborne pathogens of public health importance that cause diarrhea, hemorrhagic colitis and hemolytic uremic syndrome in humans. The main virulence factors of STEC are Shiga toxins (Stx1 and Stx2) and additionally they can also synthesize the adhesin intimin (encoded by eae), an enterohemolysin (EhxA), and an autoagglutinating protein (Saa), among other virulence factors. Cattle and sheep constitute natural reservoirs of these STEC strains and transmission to humans can happen through direct contact with infected animals and consumption of contaminated food and water.
Escherichia coli Typing stx1 & stx2 (STEC) qPCR Kit is designed for the in vitro detection of STEC genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to STEC. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of these species. The primers and probe sequences have very high (>95%) homology with a broad range of STEC genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected.
This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.
If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Vet, Food & Pharma and Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Escherichia coli Typing stx1 & stx2 (STEC) qPCR Kit is designed for the in vitro detection of STEC genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to STEC. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of these species. The primers and probe sequences have very high (>95%) homology with a broad range of STEC genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected.
This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.
If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Vet, Food & Pharma and Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Escherichia coli Typing stx1 & stx2 (STEC) |
Sample Material | DNA |
Protocol time | Approx. 60 min (from extracted NA to result) |
Number of targets | Triplex |
Internal Control | No |
Detection Method | Probe Based |
- Lyo NZYSupreme qPCR master mix (2x)
- qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- DNA Internal Extraction Control (IEC)
- qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- DNA Internal Extraction Control (IEC)
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Escherichia coli Typing stx1 & stx2 (STEC) |
Sample Material | DNA |
Protocol time | Approx. 60 min (from extracted NA to result) |
Number of targets | Triplex |
Internal Control | No |
Detection Method | Probe Based |
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Escherichia coli Typing stx1 & stx2 (STEC) |
Sample Material | DNA |
Protocol time | Approx. 60 min (from extracted NA to result) |
Number of targets | Triplex |
Internal Control | No |
Detection Method | Probe Based |
MSDS
Material Safety Data Sheets
CoA
Certificate of Analysis