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SKU
MD06301
Mannheimia haemolytica qPCR Kit
Designed for the in vitro detection of M. haemolytica. qPCR assay criteria are met, but PC is not meant for quantification.
Availability
Ready to Ship
Mannheimia haemolytica is a Gram-negative bacterium that belong to the Pasteurellaceae family. This bacterium is present in the natural flora of the upper respiratory system of ruminant. It is considered an opportunistic pathogen and the main factor of pneumonic pasteurellosis, which has caused a severe economic loss in sheep and cattle industries. M. haemolytica is the causative agent of pneumonia, septicemia and gangrenous mastitis in sheep, goats and ewes, respectively.
Mannheimia haemolytica qPCR Kit is designed for the in vitro detection of M. haemolytica genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to M. haemolytica. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of M. haemolytica genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected.
This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.
If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Mannheimia haemolytica qPCR Kit is designed for the in vitro detection of M. haemolytica genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to M. haemolytica. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of M. haemolytica genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected.
This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.
If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Mannheimia haemolytica |
Sample Material | DNA |
Protocol time | Approx. 60 min. (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Detection Method | Probe Based |
- Lyo NZYSupreme qPCR master mix (2x)
- qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- DNA Internal Extraction Control (IEC)
- qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- DNA Internal Extraction Control (IEC)
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Mannheimia haemolytica |
Sample Material | DNA |
Protocol time | Approx. 60 min. (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Detection Method | Probe Based |
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85 °C to -15 °C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Mannheimia haemolytica |
Sample Material | DNA |
Protocol time | Approx. 60 min. (from extracted NA to result) |
Number of targets | Duplex |
Internal Control | No |
Detection Method | Probe Based |
MSDS
Material Safety Data Sheets
CoA
Certificate of Analysis