Leptospira spp. (16S rDNA) qPCR Kit

Leptospira is a Gram-negative bacterium that belongs to the Leptospiraceae family. Leptospirosis is a worldwide zoonosis affecting many wild and domestic animals. Common clinical signs include fever, vomiting, abdominal pain, diarrhea, severe weakness and depression, stiffness, severe muscle pain and inability to have offspring. The disease causes economic losses as it affects livestock, with decreased milk production and death. Humans can be infected by contact with the urine of infected animals.

Leptospira spp. (16S rDNA) qPCR Kit is designed for the in vitro detection of pathogenic and intermediately pathogenic Leptospira genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to this species. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of Leptospira species. The primers and probe sequences have very high (>95%) homology with a broad range of Leptospira genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related genus are not detected.

This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used.

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see Vet, Food & Pharma and Human). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@NZYtech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.