Lyo T4 DNA Ligase 50 U/µL

NZYtech’s Lyo T4 DNA Ligase (50 U/µL) is a freeze-dried formulation embodying an ultrapure recombinant enzyme, isolated from Escherichia coli. The Lyo T4 DNA Ligase is specialized in catalysing the formation of phosphodiester bonds between abutting 5'-phosphate and 3'-hydroxyl termini in duplex DNA or RNA structures.

Meticulously engineered to provide not only an elevated purity but also a high-concentration format, this freeze-dried enzyme is remarkably furnished at a concentration that is ≈10x superior to standard T4 DNA Ligases. The enzyme is optimized for applications that necessitate stringent quality and performance, such as high-complexity library cloning and Next-Generation Sequencing (NGS) library preparations.

Lyo T4 DNA Ligase joins both blunt and cohesive-end restriction fragments of duplex DNA or RNA and repairs single-strand nicks in duplex DNA. Specially designed to expedite your workflows, this enzyme is formulated to instantaneously ligate up to 250 ng of DNA at ambient laboratory conditions (20-25°C). Remarkably, the enzyme can facilitate efficient ligation in a notably brisk timeframe of 20 seconds to 5 minutes at room temperature, streamlining and enhancing the efficiency of your molecular biology projects.

The Lyo T4 DNA Ligase is supplied with a meticulously optimized 4× Reaction Buffer to assure optimal enzymatic activity, streamline the process, and aid in achieving consistent and reliable results. Additionally, an appropriate resuspension buffer is provided for the complete reconstitution of the enzyme in just a few seconds.