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Cellulase 5C, Ruminococcus flavefaciens
Storage Conditions:
-30°C to -15°C
Cellulase 5C (RfCel5C), assigned the E.C. number 3.2.1.4, is a derivative of Ruminococcus flavefaciens. It is an endo-1,4-β-glucanase. The recombinant RfCel5C, purified from Escherichia coli, is a modular Glycoside Hydrolase family 5 (GH5) enzyme, with a C-terminal dockerin (see more details at www.cazy.org).
Availability
Produced On Demand
Cellulase 5C (RfCel5C), assigned the E.C. number 3.2.1.4, is a derivative of Ruminococcus flavefaciens. It is an endo-1,4-β-glucanase. The recombinant RfCel5C, purified from Escherichia coli, is a modular Glycoside Hydrolase family 5 (GH5) enzyme, with a C-terminal dockerin (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 0,5 mg/mL. Bulk quantities of this product can be made available upon request. To place an order, simply visit our website. We offer fast and secure shipping worldwide.
| Shipping Conditions | Shipping: Dry Ice to RT |
|---|---|
| Storage Conditions | -30°C to -15°C |
| Class | Glycoside Hydrolase |
| Activity | Cellulases |
| Family | GH5 |
| EC number | 3.2.1.4 |
| Organism | Ruminococcus flavefaciens |
| Architecture | GH5-Doc |
| Optimal pH | 6.8 |
| Optimal Temperature | 37°C |
| Substrates | 1,3-1,4-β-glucans, 1,4-β-glucans, glucomannan, xyloglucan |
Rincon et al. (2003) J Bacteriol. 185(3):703-13.
Jindou et al. (2006) J. Bacteriol. 188,7971-7976.
Miller et al. (2009) PLoS One. 4(8):e6650.
Rincon et al. (2010) PLoS One. 5(8):e12476.
Dassa et al. (2014) PLoS One. 9(7):e99221.
Israeli-Ruimy et al. (2017) Sci Rep. 7:42355.
Jindou et al. (2006) J. Bacteriol. 188,7971-7976.
Miller et al. (2009) PLoS One. 4(8):e6650.
Rincon et al. (2010) PLoS One. 5(8):e12476.
Dassa et al. (2014) PLoS One. 9(7):e99221.
Israeli-Ruimy et al. (2017) Sci Rep. 7:42355.
| Shipping Conditions | Shipping: Dry Ice to RT |
|---|---|
| Storage Conditions | -30°C to -15°C |
| Class | Glycoside Hydrolase |
| Activity | Cellulases |
| Family | GH5 |
| EC number | 3.2.1.4 |
| Organism | Ruminococcus flavefaciens |
| Architecture | GH5-Doc |
| Optimal pH | 6.8 |
| Optimal Temperature | 37°C |
| Substrates | 1,3-1,4-β-glucans, 1,4-β-glucans, glucomannan, xyloglucan |
| Shipping Conditions | Shipping: Dry Ice to RT |
|---|---|
| Storage Conditions | -30°C to -15°C |
| Class | Glycoside Hydrolase |
| Activity | Cellulases |
| Family | GH5 |
| EC number | 3.2.1.4 |
| Organism | Ruminococcus flavefaciens |
| Architecture | GH5-Doc |
| Optimal pH | 6.8 |
| Optimal Temperature | 37°C |
| Substrates | 1,3-1,4-β-glucans, 1,4-β-glucans, glucomannan, xyloglucan |
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