Chlamydia spp. qPCR Kit

Chlamydia spp. are obligate intracellular bacteria from Chlamydiaceae family and are responsible for a range of infections in humans and animals. Chlamydia spp. qPCR Kit is designed for the in vitro detection of the following species: Chlamydia muridarum, Chlamydia suis, Chlamydia trachomatis, Chlamydophila abortus, Chlamydophila caviae, Chlamydophila felis, Chlamydia pecorum, Chlamydophila pneumoniae and Chlamydophila psittaci.

The kit is built to have the broadest possible detection profile whilst remaining specific to these species. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of these species. The primers and probe sequences have very high (>95%) homology with a broad range of Chlamydia genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database at the time of design. This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes.

We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used. If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see https://nzytech.com/en/molecular-diagnostics/). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.