Clostridium difficile (toxins A and B) qPCR Kit

Clostridium difficile is a spore-forming gram-positive bacterium known for causing antibiotic-associated diarrhea and colitis. This bacterium is able to infect pigs, calves and also humans, particularly humans with compromised immune systems or those who have recently taken antibiotics. C. difficile is transmitted from person or animal to person by the fecal-oral route, shed in feces. Clostridium difficile (toxins A and B) qPCR Kit is designed for the in vitro detection of Clostridium difficile genomes.

The kit is built to have the broadest possible detection profile whilst remaining specific to C. difficile. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of C. difficile genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database at the time of design. This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes.

We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used. If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see https://nzytech.com/en/molecular-diagnostics/). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.