Ehrlichia species qPCR Kit

Ehrlichia species are obligate intracellular bacteria primarily transmitted by ticks, causing diseases such as ehrlichiosis in humans and animals. These bacteria infect white blood cells, leading to symptoms ranging from mild flu-like illness to severe manifestations such as fever, headache, and in some cases, life-threatening complications. Ehrlichia species qPCR Kit is designed for the in vitro detection of the following species: E. canis, E. ruminatium, E. chaffeensis, E. ewingii, E.muris, E. minasensis and E. ovina.

The kit is built to have the broadest possible detection profile whilst remaining specific these species. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of the previously mentioned species. The primers and probe sequences have very high (>95%) homology with a broad range of Ehrlichia spp. genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database at the time of design. This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes.

We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used. If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see https://nzytech.com/en/molecular-diagnostics/). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.