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SKU
MD06641
Influenza A virus H5N1 RT-qPCR Kit
Designed for the in vitro detection of Influenza A H5N1 virus from clades 2.3.4.4b and 2.3.2.1a. qPCR assay criteria are met, but PC is not meant for quantification.
Availability
Ready to Ship
Influenza A subtype H5N1 is a highly pathogenic avian influenza virus that primarily infects birds, particularly waterfowl, and has caused sporadic but severe outbreaks in poultry populations. This virus is of concern due to its potential to infect humans, causing severe respiratory illness, and has raised pandemic fears because of its high mortality rate when transmitted to people. Influenza A virus H5N1 RT-qPCR Kit is designed for the in vitro detection of Influenza A virus subtype H5N1 from clades 2.3.4.4b and 2.3.2.1a.
The kit is built to have the broadest possible detection profile whilst remaining specific to Influenza A virus subtype H5N1. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this subtype. The primers and probe sequences have very high (>95%) homology with a broad range of Influenza A virus subtype H5N1 genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI and OpenFlu databases at the time of design. Due to the inherent instability of RNA viral genomes, it is not possible to guarantee the detection of all clinical isolates. This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes.
We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic RNA standards can also be used. If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see https://nzytech.com/en/molecular-diagnostics/). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
The kit is built to have the broadest possible detection profile whilst remaining specific to Influenza A virus subtype H5N1. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this subtype. The primers and probe sequences have very high (>95%) homology with a broad range of Influenza A virus subtype H5N1 genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI and OpenFlu databases at the time of design. Due to the inherent instability of RNA viral genomes, it is not possible to guarantee the detection of all clinical isolates. This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes.
We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic RNA standards can also be used. If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see https://nzytech.com/en/molecular-diagnostics/). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85°C to -15°C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Influenza A virus H5N1 |
Sample Material | RNA |
Protocol time | Approx. 60 min. (from extracted NA to result) |
Number of targets | Triplex |
Internal Control | No |
Detection Method | Probe Based |
- Lyo NZYSupreme Multiplex One-Step RT-qPCR Master Mix (2x)
- Multiplex qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- RNA Internal Extraction Control (IEC)
- Multiplex qPCR master mix reconstitution buffer
- Lyo PPMix (10x)
- NTC
- Positive Control
- RNA Internal Extraction Control (IEC)
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85°C to -15°C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Influenza A virus H5N1 |
Sample Material | RNA |
Protocol time | Approx. 60 min. (from extracted NA to result) |
Number of targets | Triplex |
Internal Control | No |
Detection Method | Probe Based |
Shipping Conditions | Room Temperature |
---|---|
Storage Conditions | -85°C to -15°C |
Format | Lyophilized |
Product Category | Predesigned qPCR assays |
Compatibility | All standard Real-time thermal cyclers |
Target Species | Influenza A virus H5N1 |
Sample Material | RNA |
Protocol time | Approx. 60 min. (from extracted NA to result) |
Number of targets | Triplex |
Internal Control | No |
Detection Method | Probe Based |
CoA
Certificate of Analysis