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Anhydrosialidase 33B, Streptococcus pneumoniae

Storage Conditions:
-30°C to -15°C
Anhydrosialidase 33B (SpNan33B), assigned the E.C. number 4.2.2.15, is a derivative of Streptococcus pneumoniae. It is an enzyme that participates in the elimination of alpha-sialyl groups in N-acetylneuraminic acid glycosides, releasing 2,7-anhydro-alpha-N-acetylneuraminate. The recombinant SpNan33B, purified from Escherichia coli, is a modular Glycoside Hydrolase family 33 (GH33) enzyme, with an N-terminal family 40 Carbohydrate Binding Module (CBM40) and two GH33 catalytic module repeats (see more details at www.cazy.org).
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Anhydrosialidase 33B (SpNan33B), assigned the E.C. number 4.2.2.15, is a derivative of Streptococcus pneumoniae. It is an enzyme that participates in the elimination of alpha-sialyl groups in N-acetylneuraminic acid glycosides, releasing 2,7-anhydro-alpha-N-acetylneuraminate. The recombinant SpNan33B, purified from Escherichia coli, is a modular Glycoside Hydrolase family 33 (GH33) enzyme, with an N-terminal family 40 Carbohydrate Binding Module (CBM40) and two GH33 catalytic module repeats (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. To place an order, simply visit our website. We offer fast and secure shipping worldwide.
More Information
Shipping Conditions Shipping: Dry Ice to RT
Storage Conditions -30°C to -15°C
Class Glycoside Hydrolase
Activity Sialidases
Family GH33
EC number 4.2.2.15
Organism Streptococcus pneumoniae
Architecture CBM40-GH33a-GH33b
Optimal pH 7
Optimal Temperature 37°C
Substrates glycoprotein human alpha-1 (AGP), sialic acids
Berry et al. (1996) J Bacteriol. 178(16):4854-60.
Gut et al. (2008) FEBS Lett. 582(23-24):3348-52.
More Information
Shipping Conditions Shipping: Dry Ice to RT
Storage Conditions -30°C to -15°C
Class Glycoside Hydrolase
Activity Sialidases
Family GH33
EC number 4.2.2.15
Organism Streptococcus pneumoniae
Architecture CBM40-GH33a-GH33b
Optimal pH 7
Optimal Temperature 37°C
Substrates glycoprotein human alpha-1 (AGP), sialic acids
More Information
Shipping Conditions Shipping: Dry Ice to RT
Storage Conditions -30°C to -15°C
Class Glycoside Hydrolase
Activity Sialidases
Family GH33
EC number 4.2.2.15
Organism Streptococcus pneumoniae
Architecture CBM40-GH33a-GH33b
Optimal pH 7
Optimal Temperature 37°C
Substrates glycoprotein human alpha-1 (AGP), sialic acids

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