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Chondroitin AC lyase 8A, Bacteroides stercoris
Chondroitin AC lyase 8A (BsCac8A), assigned the E.C. number 4.2.2.5, is a derivative of Bacteroides stercoris. It is an enzyme that participates in the eliminative degradation of polysaccharides containing 1,4-β-D-hexosaminyl and 1,3-β-D-glucuronosyl linkages to disaccharides containing 4-deoxy-β-D-gluc-4-enuronosyl groupsanhydrobond in the MurNAc residue. The recombinant BsCac8A, purified from Escherichia coli, is a single-domain Pectate Lyase family 8 (PL8) enzyme (see more details at www.cazy.org).
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Produced On Demand
Chondroitin AC lyase 8A (BsCac8A), assigned the E.C. number 4.2.2.5, is a derivative of Bacteroides stercoris. It is an enzyme that participates in the eliminative degradation of polysaccharides containing 1,4-β-D-hexosaminyl and 1,3-β-D-glucuronosyl linkages to disaccharides containing 4-deoxy-β-D-gluc-4-enuronosyl groupsanhydrobond in the MurNAc residue. The recombinant BsCac8A, purified from Escherichia coli, is a single-domain Pectate Lyase family 8 (PL8) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 0,25 mg/mL. Bulk quantities of this product can be made available upon request. To place an order, simply visit our website. We offer fast and secure shipping worldwide.
Shipping Conditions | Shipping: Dry Ice to RT |
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Storage Conditions | -30°C to -15°C |
Class | Polysaccharide Lyase |
Activity | Chondroitin lyases |
Family | PL8 |
EC number | 4.2.2.5 |
Organism | Bacteroides stercoris |
Architecture | PL8 |
Optimal pH | 5.8 |
Optimal Temperature | 45°C |
Substrates | chondroitin sulfate A (CS-A), chondroitin sulfate C (CS-C), hyaluronic acid (hyaluronan) |
Shim and Kim. (2008) Protein Expr Purif. 58(2):222-8.
Shipping Conditions | Shipping: Dry Ice to RT |
---|---|
Storage Conditions | -30°C to -15°C |
Class | Polysaccharide Lyase |
Activity | Chondroitin lyases |
Family | PL8 |
EC number | 4.2.2.5 |
Organism | Bacteroides stercoris |
Architecture | PL8 |
Optimal pH | 5.8 |
Optimal Temperature | 45°C |
Substrates | chondroitin sulfate A (CS-A), chondroitin sulfate C (CS-C), hyaluronic acid (hyaluronan) |
Shipping Conditions | Shipping: Dry Ice to RT |
---|---|
Storage Conditions | -30°C to -15°C |
Class | Polysaccharide Lyase |
Activity | Chondroitin lyases |
Family | PL8 |
EC number | 4.2.2.5 |
Organism | Bacteroides stercoris |
Architecture | PL8 |
Optimal pH | 5.8 |
Optimal Temperature | 45°C |
Substrates | chondroitin sulfate A (CS-A), chondroitin sulfate C (CS-C), hyaluronic acid (hyaluronan) |
CoA
Certificate of Analysis