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Heparin-sulfate lyase 12A, Pedobacter heparinus
Heparin-sulfate lyase 12A (PhHep12A), assigned the E.C. number 4.2.2.8, is a derivative of Pedobacter heparinus. It is an enzyme that participates in the eliminative cleavage of Heparan sulfate by putativaly acting on the linkages between acetyl-glucosamine and uronate. The recombinant PhHep12A, purified from Escherichia coli, is a single-domain Pectate Lyase family 12 (PL12) enzyme (see more details at www.cazy.org).
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Produced On Demand
Heparin-sulfate lyase 12A (PhHep12A), assigned the E.C. number 4.2.2.8, is a derivative of Pedobacter heparinus. It is an enzyme that participates in the eliminative cleavage of Heparan sulfate by putativaly acting on the linkages between acetyl-glucosamine and uronate. The recombinant PhHep12A, purified from Escherichia coli, is a single-domain Pectate Lyase family 12 (PL12) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 0,5 mg/mL. Bulk quantities of this product can be made available upon request. To place an order, simply visit our website. We offer fast and secure shipping worldwide.
| Shipping Conditions | Shipping: Dry Ice to RT |
|---|---|
| Storage Conditions | -30°C to -15°C |
| Class | Polysaccharide Lyase |
| Activity | Heparin lyases |
| Family | PL12 |
| EC number | 4.2.2.8 |
| Organism | Pedobacter heparinus |
| Architecture | PL12 |
| Optimal pH | 7.5 |
| Optimal Temperature | 35°C |
| Substrates | heparan sulfate |
Godavarti et al. (1996) Biochem Biophys Res Commun. 225(3):751-8.
Su et al. (1996) Appl Environ Microbiol. 62(8):2723-34.
Hashimoto et al. (2014) Biochemistry. 53 (4), pp 777–786.
Su et al. (1996) Appl Environ Microbiol. 62(8):2723-34.
Hashimoto et al. (2014) Biochemistry. 53 (4), pp 777–786.
| Shipping Conditions | Shipping: Dry Ice to RT |
|---|---|
| Storage Conditions | -30°C to -15°C |
| Class | Polysaccharide Lyase |
| Activity | Heparin lyases |
| Family | PL12 |
| EC number | 4.2.2.8 |
| Organism | Pedobacter heparinus |
| Architecture | PL12 |
| Optimal pH | 7.5 |
| Optimal Temperature | 35°C |
| Substrates | heparan sulfate |
| Shipping Conditions | Shipping: Dry Ice to RT |
|---|---|
| Storage Conditions | -30°C to -15°C |
| Class | Polysaccharide Lyase |
| Activity | Heparin lyases |
| Family | PL12 |
| EC number | 4.2.2.8 |
| Organism | Pedobacter heparinus |
| Architecture | PL12 |
| Optimal pH | 7.5 |
| Optimal Temperature | 35°C |
| Substrates | heparan sulfate |
CoA
Certificate of Analysis
