We use cookies to make your experience better. To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
Only %1 left
SKU
Rhamnogalacturonan endolyase 9B, Bacteroides thetaiotaomicron
Rhamnogalacturonan endolyase 9B (BtRge9B), assigned the E.C. number 4.2.2.23, is a derivative of Bacteroides thetaiotaomicron. It is an enzyme that participates in the endotype eliminative cleavage of L-α-rhamnopyranosyl-1,4-α-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end. The recombinant BtRge9B, purified from Escherichia coli, is a single-domain Pectate Lyase family 9 (PL9) enzyme (see more details at www.cazy.org).
Availability
Produced On Demand
Rhamnogalacturonan endolyase 9B (BtRge9B), assigned the E.C. number 4.2.2.23, is a derivative of Bacteroides thetaiotaomicron. It is an enzyme that participates in the endotype eliminative cleavage of L-α-rhamnopyranosyl-1,4-α-D-galactopyranosyluronic acid bonds of rhamnogalacturonan I domains in ramified hairy regions of pectin leaving L-rhamnopyranose at the reducing end and 4-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the non-reducing end. The recombinant BtRge9B, purified from Escherichia coli, is a single-domain Pectate Lyase family 9 (PL9) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. To place an order, simply visit our website. We offer fast and secure shipping worldwide.
Shipping Conditions | Shipping: Dry Ice to RT |
---|---|
Storage Conditions | -30°C to -15°C |
Class | Polysaccharide Lyase |
Activity | Rhamnogalacturonan lyases |
Family | PL9 |
EC number | 4.2.2.23 |
Organism | Bacteroides thetaiotaomicron |
Architecture | PL9 |
Optimal pH | 7.5 |
Optimal Temperature | 37°C |
Substrates | rhamnogalacturonan I |
Xu et al. (2003) Science. 299(5615):2074-6.
Mahowald et al. (2009) Proc Natl Acad Sci U S A. 106(14):5859-64.
Luis et al. (2018) Nat Microbiol. 3(2): 210–219.
Mahowald et al. (2009) Proc Natl Acad Sci U S A. 106(14):5859-64.
Luis et al. (2018) Nat Microbiol. 3(2): 210–219.
Shipping Conditions | Shipping: Dry Ice to RT |
---|---|
Storage Conditions | -30°C to -15°C |
Class | Polysaccharide Lyase |
Activity | Rhamnogalacturonan lyases |
Family | PL9 |
EC number | 4.2.2.23 |
Organism | Bacteroides thetaiotaomicron |
Architecture | PL9 |
Optimal pH | 7.5 |
Optimal Temperature | 37°C |
Substrates | rhamnogalacturonan I |
Shipping Conditions | Shipping: Dry Ice to RT |
---|---|
Storage Conditions | -30°C to -15°C |
Class | Polysaccharide Lyase |
Activity | Rhamnogalacturonan lyases |
Family | PL9 |
EC number | 4.2.2.23 |
Organism | Bacteroides thetaiotaomicron |
Architecture | PL9 |
Optimal pH | 7.5 |
Optimal Temperature | 37°C |
Substrates | rhamnogalacturonan I |
CoA
Certificate of Analysis