Tris-Acetate-EDTA (TAE) Buffer 50x, pH 8.3, 50x

TAE is advantageous for high resolution of long nucleic acid fragments (longer than 1500 bp) on agarose gels. It has a lower buffering capacity than TBE and in general, nucleic acid fragments move slower in TAE gels (apart from linear dsDNA, which tends to run faster). TAE is also used for native (non-denaturing) RNA analysis and in denaturing gels (instead of MOPS buffer) using prior denaturation of the RNA samples in hot formamide.

Specifications:
-Light sensitive
-TRIS 2.0 M 242.0 g/L
-Acetic Acid 1.0 M 60.0 g/L
-Na₂EDTA 0.05 M 30.3 g/L
-RNAse Free Solution
-Final concentration is 40 mM Tris base, 20 mM Glacial Acetic Acid and 1 mM Na₂EDTA

Directions for use: Dilute 20 mL TAE Buffer 50x with 980 mL deionised water to make 1 litre of TAE Buffer 50x

Suitable for:
-Nucleic acid electrophoresis running buffer for agarose and polyacrylamide gels
-Native and denaturating RNA analysis
-Northern blotting