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NZY Bacterial Cell Lysis Buffer
NZY Bacterial Cell Lysis Buffer is an innovative product developed by NZYtech for the gentle disruption of Escherichia coli cell wall, generating a homogeneous cell-free extract.
NZY Bacterial Cell Lysis Buffer is an innovative product developed by NZYtech for the gentle disruption of Escherichia coli cell wall, generating a homogeneous cell-free extract. It provides a rapid and cost-effective alternative to harsh chemicals or mechanical procedures, such as French Press or sonication, to release recombinant and native proteins without denaturation. This extraction reagent is a Tris-buffered formulation (pH 7.5) with lysozyme and DNase I provided separately. The addition of these enzymes allows a most efficient extraction.
In, protein extraction efficiencies are strain-dependent. NZY Bacterial Cell Lysis Buffer can be used to disrupt most common bacterial host strains and it is especially efficient with BL21 strain and its derivatives. The use of pLysS or pLysE hosts enhances protein extraction efficacies.
In
| Shipping Conditions | Room Temperature | Storage Conditions | Part 1/1 (Buffer) 2 °C to 25 °C; Part 2/2 (DNase I and Lysozyme) -85 °C to -15 °C | Application | Cell Lysis | Product Category | Cell preparation & Growth |
|---|
– NZY Bacterial Cell Lysis Buffer
– Lysozyme (at 50 mg/mL)
– DNase I (at 2 mg/mL)
– Lysozyme (at 50 mg/mL)
– DNase I (at 2 mg/mL)
| Shipping Conditions | Room Temperature | Storage Conditions | Part 1/1 (Buffer) 2 °C to 25 °C; Part 2/2 (DNase I and Lysozyme) -85 °C to -15 °C | Application | Cell Lysis | Product Category | Cell preparation & Growth |
|---|
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Coelho D, A. Lopes P, Cardoso V, Ponte P, Brás J, S. Madeira M, M. Alfaia C, M. Bandarra N, G. Gerken H, M. G. A. Fontes C, Prates J.
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Cell Reports, 2021.
A two-enzyme constituted mixture to improve the degradation of Arthrospira platensis microalga cell wall for monogastric diets.
Coelho D, A. Lopes P, Cardoso V, Ponte P, Brás J, S. Madeira M, M. Alfaia C, M. Bandarra N, M. G. A. Fontes C, A. M. Prates J.
Journal of Animal Physiology and Animal Nutrition, 2019.
Physiological characterization of a pyrimidine auxotroph exposes link between uracil phosphoribosyltransferase regulation and riboflavin production in Ashbya gossypii.
Silva R, Aguiar TQ, Oliveira C, Domingues L
N Biotechnol, 2019.
Recombinant protein purification and immobilization strategies based on peptides with dual affinity to iron oxide and silica.
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Biotechnology Journal, 2023.
Bare silica as an alternative matrix for affinity purification/immobilization of His-tagged proteins.
Freitas I A, Domingues L, Aguiar Q T.
Separation and Purification Technology, 2022.
| Shipping Conditions | Room Temperature | Storage Conditions | Part 1/1 (Buffer) 2 °C to 25 °C; Part 2/2 (DNase I and Lysozyme) -85 °C to -15 °C | Application | Cell Lysis | Product Category | Cell preparation & Growth |
|---|
| Shipping Conditions | Room Temperature | Storage Conditions | Part 1/1 (Buffer) 2 °C to 25 °C; Part 2/2 (DNase I and Lysozyme) -85 °C to -15 °C | Application | Cell Lysis | Product Category | Cell preparation & Growth |
|---|
SDS
Safety Data Sheets
CoA
Certificate of Analysis
