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SKU
MB42501
T7 DNA Ligase
T7 DNA Ligase is a typical DNA ligase that catalyses the formation of a phosphodiester bond between adjacent 5´ phosphate and 3´ hydroxyl groups of duplex DNA molecules.
Availability
Ready to Ship
Dry Ice
T7 DNA Ligase is a typical DNA ligase that catalyses the formation of a phosphodiester bond between adjacent 5´ phosphate and 3´ hydroxyl groups of duplex DNA molecules. Cohesive end ligation and nick sealing are efficiently catalysed by T7 DNA Ligase, although the enzyme does not perform blunt end ligations. Thus, the enzyme discriminates between cohesive (active) and blunt (not active) fragment DNA ligations, as it only performs sticky end ligations. T7 DNA Ligase requires ATP for activity.
Applications:
– Cloning of DNA fragments generated by restriction enzymes digestion
– Nick-sealing in dsDNA
– Linear DNA circularization
Applications:
– Cloning of DNA fragments generated by restriction enzymes digestion
– Nick-sealing in dsDNA
– Linear DNA circularization
| Shipping Conditions | Dry Ice |
|---|---|
| Storage Conditions | -85 °C to -15 °C |
| Activity | Sticky-end ligation |
| Product Category | Ligases |
| Reaction Temperature | 16 °C |
| Heat Inactivation | No |
| Concentration | 3000 U/μL |
– T7 DNA Ligase (3000 U/μL)
– Reaction buffer (4x)
– Reaction buffer (4x)
| Shipping Conditions | Dry Ice |
|---|---|
| Storage Conditions | -85 °C to -15 °C |
| Activity | Sticky-end ligation |
| Product Category | Ligases |
| Reaction Temperature | 16 °C |
| Heat Inactivation | No |
| Concentration | 3000 U/μL |
| Shipping Conditions | Dry Ice |
|---|---|
| Storage Conditions | -85 °C to -15 °C |
| Activity | Sticky-end ligation |
| Product Category | Ligases |
| Reaction Temperature | 16 °C |
| Heat Inactivation | No |
| Concentration | 3000 U/μL |
CoA
Certificate of Analysis
