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NZYSpeedy Miniprep
NZYSpeedy Miniprep kit is designed for the ultra-rapid preparation of highly pure plasmid DNA from E. coli strains.
NZYSpeedy Miniprep kit is designed for the ultra-rapid, small-scale preparation of highly pure plasmid DNA from recombinant Escherichia colistrains. NZYtech´s speedy miniprep procedure is based on the alkaline lysis of bacterial cells followed by adsorption of DNA onto a new specially treated silica membrane in the presence of high salt. The plasmid DNA is selectively adsorbed in the silica gel-based NZYtech plasmid spin column and other impurities such as proteins, salts, nucleotides and oligos (<40-mer) are washed away. The eluted DNA is suitable for applications like automated fluorescent sequencing, PCR and a wide range of other enzymatic manipulations. NZYSpeedy Miniprep kit includes a newly developed washing buffer (AS) which ensures complete removal of contaminants in a rapid washing step.
| Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | In Vitro transcription, Cloning, PCR, Sequencing, Transformation | Starting Material | E. coli culture | Scale | Miniprep | Features | Endonuclease Removal, Fast, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number | Format | Column | Product Category | DNA Purification, Plasmid DNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 15 min. |
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– Buffer A1
– Buffer A2
– Buffer A3
– Buffer AS (concentrate)
– Buffer AE (does not contain EDTA)
– RNase A
– NZYTech Spin Columns
– Collection Tubes (2 mL)
– Buffer A2
– Buffer A3
– Buffer AS (concentrate)
– Buffer AE (does not contain EDTA)
– RNase A
– NZYTech Spin Columns
– Collection Tubes (2 mL)
| Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | In Vitro transcription, Cloning, PCR, Sequencing, Transformation | Starting Material | E. coli culture | Scale | Miniprep | Features | Endonuclease Removal, Fast, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number | Format | Column | Product Category | DNA Purification, Plasmid DNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 15 min. |
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Genetic diversity, phylogenetic position, and co-phylogenetic relationships of Karyolysus, a common blood parasite of lizards in the western Mediterranean.
Megía-Palma R, Martínez J, Fitze P, Cuervo J, Belliure J, Jiménez-Robles O, Cabido C, Martín J, Merino S.
Journal of Parasitology (Elsevier), 2023.
The Construction of an Environmentally Friendly Super-Secreting Strain of Bacillus subtilis through Systematic Modulation of Its Secretory Pathway Using the CRISPR-Cas9 System.
Ferrando J, Miñana-Galbis D, Pere Picart P.
International Journal of Molecular Sciences (IJMS), 2024.
Epigenetic Regulation of ZNF687 by miR-142a-3p and DNA Methylation During Osteoblast Differentiation and Mice Bone Development and Aging.
Varela D, Varela T, Conceição N, Cancela L.
International Journal of Molecular Sciences (IJMS), 2025.
Visual Nanoprobe-Enhanced Loop-Mediated Isothermal Amplification Protocol for Rapid Detection of Infectious Laryngotracheitis Virus from Avian Respiratory Swabs.
Cea-Callejo P, Trenado C, Doménech A, Madrid R, Benítez L.
Bio Protoc, 2026.
Exploiting Co(III)-Cyclopentadienyl Complexes To Develop Anticancer Agents.
Franco Machado J, Cordeiro S, Duarte J, Costa P, Mendes P, Garcia H, Baptista P, Fernandes A, Morais T.
Inorganic Chemistry, 2024.
| Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | In Vitro transcription, Cloning, PCR, Sequencing, Transformation | Starting Material | E. coli culture | Scale | Miniprep | Features | Endonuclease Removal, Fast, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number | Format | Column | Product Category | DNA Purification, Plasmid DNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 15 min. |
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Plasmid DNA was isolated from 3 mL of a recombinant E. coli strain using three different rapid protocols (NZYTech, lanes 1-4; Competitor 1, lanes 5-8; Competitor 2, lanes 9-12). DNA isolation was performed according to each manufacturer ́s protocol. The quadruplicates (2 μL of 50 μL eluate) were analysed through agarose gel electrophoresis (A). Lane M: NZYDNA Ladder III. Eluates were also analysed by UV/VIS spectroscopy to determine total yields (B).
Protocol Overview:
Protocol Overview:
| Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | In Vitro transcription, Cloning, PCR, Sequencing, Transformation | Starting Material | E. coli culture | Scale | Miniprep | Features | Endonuclease Removal, Fast, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number | Format | Column | Product Category | DNA Purification, Plasmid DNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 15 min. |
|---|
| Shipping Conditions | Room Temperature | Storage Conditions | 15 °C to 25 °C | Application | In Vitro transcription, Cloning, PCR, Sequencing, Transformation | Starting Material | E. coli culture | Scale | Miniprep | Features | Endonuclease Removal, Fast, Plasmid type: BACs, cosmids, Plasmid type: high-, low-copy number | Format | Column | Product Category | DNA Purification, Plasmid DNA Purification | Isolation Technology | Silica membrane | Protocol time | Approx. 15 min. |
|---|
SDS
Safety Data Sheets
CoA
Certificate of Analysis
