NZY DNA/RNA/Protein Isolation kit

The NZY DNA/RNA/Protein Isolation Kit overcomes challenges in simultaneous DNA, RNA, and protein isolation from small and diverse samples. These samples may include biopsies, tumors, tissues, and transgenic organisms. This kit does not require splitting the sample before extraction, thus providing DNA, RNA, and protein from the exact same portion. DNA and RNA are separately eluted with low salt buffer and water, respectively. All isolated products are suitable for common downstream applications. The NZY DNA/RNA/Protein Isolation method ensures the preservation of DNA, RNA, and protein integrity. Cells are lysed in a chaotropic ion-rich solution, inhibiting enzymes like DNases, RNases, proteases, and phosphatases, and simultaneously allowing DNA and RNA to bind to the silica membrane. This process negates the need for costly proteinase inhibitors. After two specialized washing steps, DNA is selectively eluted using a low salt buffer, without compromising RNA quality or quantity. Any residual DNA is removed by an rDNase solution applied directly to the silica membrane. After the washing steps, RNA is eluted using RNase-free water. Protein is isolated from the initial column flowthrough and precipitated with a protein precipitator buffer (Buffer NPP). The protein pellet is then dissolved in protein solving buffer (Buffer NPS) containing the odourless reducing agent TCEP, ready for SDS-PAGE analysis. Note that this kit is not suitable for the isolation of native proteins. DNA, RNA, and protein extraction can be performed at room temperature, but due to the sensitivity of RNA to trace RNase contamination, the DNA and RNA eluates require careful handling. DNA should be stored at 4 °C for short-term and -20 °C for long-term storage. To maintain RNA stability, store at -20 °C short-term or -70 °C long-term. Protein dissolved in protein solving buffer (Buffer NPS) is relatively stable.