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NZYTaq II 2x Green Master Mix
NZYTaq II 2× Green Master Mix is a premixed ready-to-use solution containing NZYTaq II DNA polymerase (MB354), which belongs to a new generation of Taq-derived DNA polymerases optimized for standard PCR applications.
NZYTaq II 2× Green Master Mix is a premixed ready-to-use solution containing NZYTaq II DNA polymerase (MB354), which belongs to a new generation of Taq-derived DNA polymerases optimized for standard PCR applications. The master mix contains dNTPs, reaction buffer and additives at optimal concentrations and supports the robust and reliable amplification of a wide range of DNA templates up to 6 kb. MgCl2 final concentration is 2.5 mM, allowing the implementation of a comprehensive range of PCR protocols. In addition, reactions assembled with NZYTaq II 2× Green Master Mix may be directly loaded onto agarose gels. The mix contains two dyes (blue and yellow) that allow monitoring the progress of the electrophoresis. NZYTaq II 2× Green Master Mix is not suitable when direct fluorescent or absorbance readings are required without prior purification of the amplified DNA from PCR. We recommend using the master mix version without dyes NZYTaq II 2× Colourless Master Mix (MB357) or purifying the PCR product using NZYGelpure (MB011) before performing such downstream protocols. NZYTaq II DNA polymerase lacks 3’→5’ exonuclease activity. Resulting PCR products have an A-overhang and are suitable for cloning with NZYtech’s NZY-A PCR cloning kit (MB053) or NZY-A Speedy PCR cloning kit (MB137).
Shipping Conditions | Room Temperature | Storage Conditions | -85°C to -15°C | Application | Colony PCR, Routine PCR, TA Cloning | Features | Standard | Product Length | 0-6 kb | Format | Mastermix | Product Category | End-Point PCR | Concentration | 0.2 U/μL | Hot Start | No |
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NZYTaq II 2× Green Master Mix (0.2 U/μL)
Shipping Conditions | Room Temperature | Storage Conditions | -85°C to -15°C | Application | Colony PCR, Routine PCR, TA Cloning | Features | Standard | Product Length | 0-6 kb | Format | Mastermix | Product Category | End-Point PCR | Concentration | 0.2 U/μL | Hot Start | No |
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Structural diversity of photoautotrophic populations within the UNESCO site ‘Old Cathedral of Coimbra’ (Portugal), using a combined approach
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Fungal diversity and distribution across distinct biodeterioration phenomena in limestone walls of the old cathedral of Coimbra, UNESCO World Heritage Site
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Costa, A, Santos A, Sousa, J, Rodrigues J, Gudiña E, Silvério C, Rodrigues L
Applied Microbiology and Biotechnology, 2021
CRISPR-Cas9-Mediated Genomic Deletions Protocol in Zebrafish
Amorim J P, Bordeira-Carriço R, Gali-Macedo A, Perrod C,
Star Prtocols CellPress, 2020
Tet3 ablation in adult brain neurons increases anxiety-like behavior and regulates cognitive function in mice
Antunes C, Da Silva JD, Alves ND, Ferreira F, Loureiro-Campos E, Branco MR, Reik W, Pinto L, Marques J
Molecular Psychiatry, 2021
Preliminary comparative analysis of the resolving power of COX1 and
16S-rDNA as molecular markers for the identification of ticks from Portugal
Filipe D, Parreira R, Pereira A, Galvão N, Cristovão MJ, Nunes M, Vieira LM, Campino L, Maia, C
Veterinary Parasitology: Regional Studies and Repors, 2021
Structural diversity of photoautotrophic populations within the UNESCO site ‘Old Cathedral of Coimbra’ (Portugal), using a combined approach
Soares F, Portugal A, Trovão J, Coelho C, Mesquita N, Pinheiro AC, Gil F, Catarino L, Cardoso SM, Tiago I
International Biodeterioration & Biodegradation, 2019
Description of Aeminiaceae fam. nov., Aeminium gen. nov. and Aeminiumludgeri sp. nov. (Capnodiales), isolated from a biodeteriorated art-piece in the Old Cathedral of Coimbra, Portugal
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Shipping Conditions | Room Temperature | Storage Conditions | -85°C to -15°C | Application | Colony PCR, Routine PCR, TA Cloning | Features | Standard | Product Length | 0-6 kb | Format | Mastermix | Product Category | End-Point PCR | Concentration | 0.2 U/μL | Hot Start | No |
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Shipping Conditions | Room Temperature | Storage Conditions | -85°C to -15°C | Application | Colony PCR, Routine PCR, TA Cloning | Features | Standard | Product Length | 0-6 kb | Format | Mastermix | Product Category | End-Point PCR | Concentration | 0.2 U/μL | Hot Start | No |
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CoA
Certificate of Analysis