Speedy Supreme NZYTaq DNA polymerase

Speedy Supreme NZYTaq DNA polymerase is a recombinant modified form of Taq DNA polymerase that combines hot-start like PCR capacity and a fast polymerization reaction. The enzyme is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer-dimers and thus providing higher specificity and sensitivity of DNA amplification. The functional activity of the enzyme is restored during a short 5-minute incubation step at 95 °C.

Only 5 seconds are required to successfully amplify a DNA fragment of 1 kb. The enzyme retains its speed when amplifying fragments up to around 2 kb. Successful amplification of higher DNA fragments up to 6 kb in size can be reached using a 10 sec/kb extension step. Faster PCR can be further achieved by increasing the primers melting temperature, which increases PCR annealing temperature, thus allowing combining the annealing and extension PCR steps during PCR cycling (see below).

Speedy Supreme NZYTaq DNA polymerase is supplied with an optimized 10× Reaction Buffer and a 50 mM MgCl₂ solution. Minimal optimizations are required for successful DNA amplification. In addition, the hot-start-like capacity of Speedy Supreme NZYTaq DNA polymerase not only leads to higher PCR sensitivity but also allows a room-temperature reaction setup. The enzyme lacks 3’→5’ exonuclease activity and the PCR products generated have an Aoverhang, being suitable for cloning with NZYtech’s TA PCR cloning kits (MB053 or MB137).