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NZY M-MuLV Reverse Transcriptase

Storage Conditions:
-85 °C to -15 °C
NZY M-MuLV Reverse Transcriptase synthesizes cDNA in the presence of a primer using either RNA or single-stranded DNA as template. The enzyme lacks 3’→5´ exonuclease activity and has no RNase H activity thus enabling improved synthesis of full-length cDNA.
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Blue Ice / Ice Packs
From €80.00
NZY M-MuLV Reverse Transcriptase is a recombinant form of the Reverse Transcriptase from the Moloney Murine Leukemia Virus (M-MuLV) purified from Escherichia coli. The enzyme synthesizes the complementary DNA strand in the presence of a primer using either RNA or single-stranded DNA as a template. NZY M-MuLV Reverse Transcriptase lacks 3’→5´ exonuclease activity and has no RNase H activity thus enabling improved synthesis of full-length cDNA, even for long mRNA, using random priming.
More Information
Shipping Conditions Blue Ice
Storage Conditions -85 °C to -15 °C
Application cDNA synthesis, RT-PCR, RT-qPCR
Features High yield
Format Enzyme/Protein, Liquid
Product Category cDNA Synthesis
Reaction Temperature 37 °C
Heat Inactivation 70 °C
Protocol time Approx. 60 min.
– NZY M– MuLV Reverse Transcriptase (200 U/μL)
– Reaction Buffer (10x)
More Information
Shipping Conditions Blue Ice
Storage Conditions -85 °C to -15 °C
Application cDNA synthesis, RT-PCR, RT-qPCR
Features High yield
Format Enzyme/Protein, Liquid
Product Category cDNA Synthesis
Reaction Temperature 37 °C
Heat Inactivation 70 °C
Protocol time Approx. 60 min.
Carbonic anhydrases are involved in mitochondrial biogenesis and control the production of lactate by human Sertoli cells
Bernardino RL, Dias TR, Moreira BP, Cunha M, Barros A, Oliveira E, Sousa M, Alves MG, Oliveira PF
FEBS J, 2019

Estrogen Modulates Glycerol Permeability in Sertoli Cells through Downregulation of Aquaporin-9
Bernardino RL, Carrageta DF, Silva AM, Calamita G, Alves MG, Soveral G, Oliveira PF
Cells, 2018

Estrogenic regulation of bicarbonate transporters from SLC4 family in rat Sertoli cells
Bernardino RL, Martins AD, Jesus TT, Sá R, Sousa M, Alves MG, Oliveira PF
Mol Cell Biochem, 2015

Testicular Metabolic Reprogramming in Neonatal Streptozotocin-Induced Type 2 Diabetic Rats Impairs Glycolytic Flux and Promotes Glycogen Synthesis
Rato L, Alves MG, Dias TR, Cavaco JE, Oliveira PF
J Diabetes Res, 2015

Metabolic fingerprints in testicular biopsies from type 1 diabetic patients
Alves MG, Martins AD, Moreira PI, Carvalho RA, Sousa M, Barros A, Silva J, Pinto S, Simões T, Oliveira PF
Cell Tissue Res, 2015

Leptin modulates human Sertoli cells acetate production and glycolytic profile: a novel mechanism of obesity-induced male infertility?
Martins AD, Moreira AC, Sá R, Monteiro MP, Sousa M, Carvalho RA, Silva BM, Oliveira PF, Alves MG
Biochem Biophys Act, 2015

Aquaporin-4 as a molecular partner of cystic fibrosis transmembrane conductance regulator in rat Sertoli cells
Jesus TT, Bernardino RL, Martins AD, Sá R, Sousa M, Alves MG, Oliveira PF
Biochem Biophys Res Commun, 2014

Aquaporin-9 is expressed in rat Sertoli cells and interacts with the cystic fibrosis transmembrane conductance regulator
Jesus TT, Bernardino RL, Martins AD, Sá R, Sousa M, Alves MG, Oliveira PF.
IUBMB Life, 2014

Copper‐induced stress in Solanum nigrum L. and antioxidant defense system responses
Fidalgo F, Azenha M, Silva AF, de Sousa A, Santiago A, Ferraz P, Teixeira J
Food and Energy Security, 2013
More Information
Shipping Conditions Blue Ice
Storage Conditions -85 °C to -15 °C
Application cDNA synthesis, RT-PCR, RT-qPCR
Features High yield
Format Enzyme/Protein, Liquid
Product Category cDNA Synthesis
Reaction Temperature 37 °C
Heat Inactivation 70 °C
Protocol time Approx. 60 min.
More Information
Shipping Conditions Blue Ice
Storage Conditions -85 °C to -15 °C
Application cDNA synthesis, RT-PCR, RT-qPCR
Features High yield
Format Enzyme/Protein, Liquid
Product Category cDNA Synthesis
Reaction Temperature 37 °C
Heat Inactivation 70 °C
Protocol time Approx. 60 min.

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