Only %1 left
SKU

NZYMutagenesis kit

Storage Conditions:
Competent cells: -85°C to -65°C
–Other components: -30°C to -15°C
NZYMutagenesis kit provides a simple and highly efficient method to generate point mutations and delete or insert single (or multiple) nucleotides in any type of plasmid DNA using PCR.
Dry Ice
NZYMutagenesis kit is designed to make point mutations and delete or insert single or multiple nucleotides in a DNA sequence. The system requires the provision of two synthetic oligonucleotide primers containing the desired mutation. Incorporation of the oligonucleotide primers with NZYProof DNA polymerase generates a mutated plasmid containing staggered nicks, which resists Dpn Idigestion (as the synthetic DNA is not methylated). The resulting mutated plasmid is recovered through transformation of NZYStar competent cells. For a schematic presentation of the mutagenesis protocol, see Figure 1. DNA isolated from dam- Escherichia coli strains, including JM101 and SCS110, is not a suitable template forthe mutagenesis reaction.
More Information
Shipping Conditions Dry Ice
Storage Conditions Competent cells: -85°C to -65°C
–Other components: -30°C to -15°C
Features Mutagenesis of plasmids <15 kb
Product Category DNA Cloning & Mutagenesis
– Reaction Buffer (10x)

– dNTP mix

– NZYProof DNA polymerase (2.5 U/µL)

– Dpn I (10 U/µL)

– Control plasmid

– Control primer mix

– NZYStar Competent Cells (*)

– Competent Cells Control Plasmid (0.1 ng/µL) (*)



(*) only provided in MB01202 kit
More Information
Shipping Conditions Dry Ice
Storage Conditions Competent cells: -85°C to -65°C
–Other components: -30°C to -15°C
Features Mutagenesis of plasmids <15 kb
Product Category DNA Cloning & Mutagenesis
A protein trisulfide couples dissimilatory sulfate reduction to energy conservation
Santos AA, Venceslau SS, Grein F, Leavitt WD, Dahl C, Johnston DT, Pereira IA
Science, 2015

Interaction studies between periplasmic cytochromes provide insights into extracellular electron transfer pathways of Geobacter sulfurreducens
Fernandes AP, Nunes TC, Paquete CM, Salgueiro CA
Biochem J, 2017

Exploring the molecular mechanisms of electron shuttling across the microbe/metal space
Paquete CM, Fonseca BM, Cruz DR, Pereira TM, Pacheco I, Soares CM, Louro RO
Front Microbiol, 2014

Ser119 phosphorylation modulates the activity and conformation of PRRXL1, a homeodomain transcription factor
Soares-Dos-Reis R, Pessoa A S, Matos M R, Falcão M, Mendes VM, Manadas B, Monteiro F A, Lima D, Reguenga C
Biochemical Journal, 2014

Dual role of Tlx3 as modulator of Prrxl1 transcription and phosphorylation
Regadas I, Soares-Dos-Reis R, Falcão M, Matos MR, Monteiro FA, Lima D, Reguenga C
Biochimica et Biophysica Acta, 2014"
More Information
Shipping Conditions Dry Ice
Storage Conditions Competent cells: -85°C to -65°C
–Other components: -30°C to -15°C
Features Mutagenesis of plasmids <15 kb
Product Category DNA Cloning & Mutagenesis
Site-directed mutagenesis
More Information
Shipping Conditions Dry Ice
Storage Conditions Competent cells: -85°C to -65°C
–Other components: -30°C to -15°C
Features Mutagenesis of plasmids <15 kb
Product Category DNA Cloning & Mutagenesis
More Information
Shipping Conditions Dry Ice
Storage Conditions Competent cells: -85°C to -65°C
–Other components: -30°C to -15°C
Features Mutagenesis of plasmids <15 kb
Product Category DNA Cloning & Mutagenesis

MSDS

Material Safety Data Sheets

CoA

Certificate of Analysis

Please insert your lot number to get the available CoA