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NcoI

Storage Conditions:
-30 °C to -15 °C
NcoI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the NcoI gene from Nocardia corallina. NcoI activity is not affected by Dam methylation, Dcm methylation or CpG methylation.
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Dry Ice
From €35.00
Restriction enzymes are used to recognize a specific sequence of nucleotides and cut double stranded DNA into smaller fragments at a specific location. Each restriction enzyme recognises different DNA sequences. NcoI is a Restriction Endonuclease purified from an Escherichia coli strain that carries the NcoI gene from Nocardia corallina. NcoI activity is not affected by Dam methylation, Dcm methylation and CpG methylation. The optimum reaction temperature is at 37 °C.

Concentration: 10000 U/mL

Reaction Buffer: NZYTech provides NcoI with a specific buffer, NZYBuffer B, and a 0.1% BSA solution. For double digestions please use 10x NZYBuffer U (MB110, not provided).

Activity in NZYTech Buffers:
NZYBuffer A: 20%
NZYBuffer B: 60%*
NZYBuffer C: (60)%
NZYBuffer U: 100%

() weak star activity is detected
* 100% activity is obtained by addition of 0.01% BSA (final concentration)

Minimize exposure of enzyme to temperatures higher than -20 °C.
More Information
Shipping Conditions Dry Ice
Storage Conditions -30 °C to -15 °C
Application DNA digestion
Features Standard
Format Liquid
Isoschizomers Bsp19I
Incubation Temperature 37 °C
Product Category Restriction Enzymes
Heat Inactivation 65 °C for 20 minutes
Protocol time Approx. 60 min
More Information
Shipping Conditions Dry Ice
Storage Conditions -30 °C to -15 °C
Application DNA digestion
Features Standard
Format Liquid
Isoschizomers Bsp19I
Incubation Temperature 37 °C
Product Category Restriction Enzymes
Heat Inactivation 65 °C for 20 minutes
Protocol time Approx. 60 min

MSDS

Material Safety Data Sheets

CoA

Certificate of Analysis

Please insert your lot number to get the available CoA

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