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Supreme NZYTaq II DNA polymerase

Storage Conditions:
-85°C to -15°C
Supreme NZYTaq II DNA polymerase is an engineered version of NZYTaq II DNA polymerase displaying a hot-start-like PCR capacity.
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From €82.00
Supreme NZYTaq II DNA polymerase is an engineered version of NZYTaq II DNA polymerase displaying a hot-start-like PCR capacity. The enzyme activity at room temperature is limited, avoiding extension of non-specifically annealed primers or primer-dimers and thus providing higher specificity, sensitivity and yield during DNA amplification. The functional activity of the enzyme is restored during a short 5-minute incubation step at 95 °C. Once Supreme NZYTaq II DNA polymerase is activated, it displays high performance leading to high DNA yields in shorter extension times (15-30 s/kb) of DNA templates up to 6 kb. Supreme NZYTaq II DNA polymerase requires minimal optimization. In addition, the hot-start-like capacity of Supreme NZYTaq II DNA polymerase not only leads to higher PCR sensitivity but also allows a room-temperature reaction setup. Supreme NZYTaq II DNA polymerase lacks 3’→5’ exonuclease activity. Thus, resulting PCR products have an A-overhang and are suitable for cloning with NZYTech’s TA PCR cloning kits (MB053 or MB137).

Extension time: 15-30 sec/kb at 72 °C

3'→5' activity: no

Product overhang: 3'-A

Fidelity (vs. Taq): 1x
More Information
Shipping Conditions Blue Ice
Storage Conditions -85°C to -15°C
Application Mouse Genotyping, Powerful PCR, TA Cloning
Features Hot-start
Product Length 0-6 kb
Format Enzyme/Protein
Product Category End-Point PCR
Concentration 5 U/μL
Hot Start Yes
– Supreme NZYTaq II DNA polymerase (5 U/μL)

– Reaction buffer (10x)

– MgCl2 Solution (50 mM)
More Information
Shipping Conditions Blue Ice
Storage Conditions -85°C to -15°C
Application Mouse Genotyping, Powerful PCR, TA Cloning
Features Hot-start
Product Length 0-6 kb
Format Enzyme/Protein
Product Category End-Point PCR
Concentration 5 U/μL
Hot Start Yes
Curcumin biosynthesis from ferulic acid by engineered Saccharomyces cerevisiae
Rainha J, Rodrigues J, Faria C, Rodrigues L
Biotechnology Journal, 2021
More Information
Shipping Conditions Blue Ice
Storage Conditions -85°C to -15°C
Application Mouse Genotyping, Powerful PCR, TA Cloning
Features Hot-start
Product Length 0-6 kb
Format Enzyme/Protein
Product Category End-Point PCR
Concentration 5 U/μL
Hot Start Yes
loader
More Information
Shipping Conditions Blue Ice
Storage Conditions -85°C to -15°C
Application Mouse Genotyping, Powerful PCR, TA Cloning
Features Hot-start
Product Length 0-6 kb
Format Enzyme/Protein
Product Category End-Point PCR
Concentration 5 U/μL
Hot Start Yes
More Information
Shipping Conditions Blue Ice
Storage Conditions -85°C to -15°C
Application Mouse Genotyping, Powerful PCR, TA Cloning
Features Hot-start
Product Length 0-6 kb
Format Enzyme/Protein
Product Category End-Point PCR
Concentration 5 U/μL
Hot Start Yes

MSDS

Material Safety Data Sheets

CoA

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Please insert your lot number to get the available CoA

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