Dirofilaria immitis qPCR Kit

Dirofilaria immitis is a parasitic roundworm commonly known as heartworm or dog heartworm. It is primarily transmitted through mosquito bites to dogs, cats, and other mammals. Although the definitive host is the dog, it can also infect cats, wolves, coyotes, jackals, foxes, ferrets, bears, seals, sea lions and, under rare circumstances, humans. Adult heartworms often reside in the pulmonary arterial system (lung arteries) as well as the heart, and a major health effect in the infected animal host is a manifestation of damage to its lung vessels and tissues. Dirofilaria immitis qPCR Kit is designed for the in vitro detection of Dirofilaria immitis genomes.

The kit is built to have the broadest possible detection profile whilst remaining specific to D. immitis. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of this species. The primers and probe sequences have very high (>95%) homology with a broad range of D. immitis genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database at the time of design. This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes.

We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic DNA standards can also be used. If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available at NZYtech (see https://nzytech.com/en/molecular-diagnostics/). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.