Mycoplasma gallisepticum & Mycoplasma synoviae qPCR Kit

Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) are significant avian respiratory pathogens that can lead to substantial economic losses in the poultry industry. Infections with these bacteria can result in reduced egg production and may affect the quality of carcasses. M. gallisepticum primarily causes chronic respiratory disease (CRD) in chickens and can also lead to infectious synovitis in chickens and turkeys. On the other hand, M. synoviae mainly causes infectious synovitis, primarily affecting the synovial membranes of joints and tendon sheaths in chickens. Horizontal transmission through respiratory aerosols and direct contact with infected birds is the predominant mode of transmission for both M. gallisepticum and M. synoviae. While transovarian transmission is possible for M. gallisepticum, it is not a common route for M. synoviae. Additionally, although less common, contaminated water and food can also contribute to transmission by indirectly exposing birds to the bacteria through feces and respiratory secretions.

Mycoplasma gallisepticum & Mycoplasma synoviae qPCR Kit is designed for the in vitro detection of both M. gallisepticum and M. synoviae genomes. The kit is built to have the broadest possible detection profile whilst remaining specific to M. gallisepticum and M. synoviae. Thus, this kit has been designed for the specific (inclusivity) and exclusive (exclusivity) in vitro detection of these species. The primers and probes sequences have very high (>95%) homology with a broad range of M. gallisepticum and M. synoviae genomes based on a comprehensive bioinformatic analysis with all reference data within the NCBI database. Other closely related species are not detected. This kit was meticulously designed and validated to meet the rigorous criteria of a quantitative assay. However, it is important to note that the provided Positive Control is not intended for quantification purposes. We recommend checking NZYtech website for the availability of a suitable Quantitative Standard for an accurate quantification. In alternative, commercially genomic RNA standards can also be used.

If required, a complementary kit for the detection of an endogenous gene of the species from which samples are being extracted is available for purchase (see Vet, Food & Pharma). The complementary usage of an Endogenous Detection reaction provides a solid confirmation that nucleic acids were properly extracted from the selected biological matrix. If you require further information or have a specific question about the detection profile of this kit, please send an e-mail to info@nzytech.com and our scientific team will answer your question. This kit is designed to be used by trained users in a suitable molecular biology laboratory environment.